Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing.

Area of Science:

• Materials Science
• Biotechnology
• Analytical Chemistry

Background:

• Biosensors utilize biological receptors and transducers for signal conversion.
• Carboxylation of graphene enhances its utility in biosensing applications.

Purpose of the Study:

• To develop a novel carboxylated graphene (CG) synthesis method.
• To create a sensitive biosensor for detecting SARS-CoV-2 IgA antibodies using CG-modified electrodes.

Main Methods:

• Carboxylation of graphene under acidic conditions.
• Characterization using SEM, EDS, Raman, TGA, and XRD.
• Immobilization of SARS-CoV-2 N-protein on CG-modified screen-printed carbon electrodes (SPCEs).
• Electrochemical analysis (cyclic voltammetry, impedance spectroscopy).

Main Results:

• Successful synthesis and characterization of carboxylated graphene.
• Demonstrated effective immobilization of SARS-CoV-2 N-protein.
• Achieved efficient electrochemical detection of IgA-SARS-CoV-2.
• Established a limit of detection at 1:1601 v/v dilution.

Conclusions:

• The developed CG-based biosensor offers a sensitive and specific method for IgA-SARS-CoV-2 detection.
• The biosensor exhibits a fast response time, requires minimal sample volume, and leverages nanomaterial signal enhancement.
• This technology holds significant potential for rapid, point-of-care diagnostic applications.