JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Circulating and Magnetic Resonance Imaging Biomarkers of Intestinal Fibrosis in Small Bowel Crohn's Disease

  • 0Department of Radiology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Inflammatory Bowel Diseases +

|

January 24, 2025

|

January 24, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Biomarkers in blood and MRI scans correlate with inflammation and fibrosis in Crohn's disease ileal resections. These findings may help guide treatment decisions for patients with refractory disease.

Area Of Science

  • Gastroenterology
  • Medical Biomarkers
  • Inflammatory Bowel Disease Research

Background

  • Previous research identified circulating and MRI biomarkers linked to Crohn's disease (CD) surgical management.
  • This study investigated the association between these biomarkers and inflammation and collagen content in ileal resections.

Purpose Of The Study

  • To determine if circulating and MRI-derived biomarkers correlate with histological inflammation and fibrosis in ileal resections from CD patients.
  • To explore the potential of these biomarkers in guiding clinical decisions for refractory CD.

Main Methods

  • Fifty CD patients undergoing ileal resection were prospectively enrolled.
  • Circulating biomarkers (CD64, ECM1, GM-CSF Ab, fecal calprotectin) and MRI parameters (3D MTR, MOLLI T1, IVIM, sMaRIA) were measured.
  • Ileal tissue inflammation and collagen content were assessed histologically and via microscopy.

Main Results

  • An inverse relationship was observed between ileal inflammation and collagen content (r = -0.39, P = .008).
  • Several biomarkers, including CD64, GM-CSF Ab, fecal calprotectin, and sMaRIA, showed associations with inflammation and collagen levels.
  • A multivariable model indicated significant associations of ECM1 and GM-CSF Ab with ileal collagen content.

Conclusions

  • Circulating and MRI biomarkers are associated with the balance of inflammation and fibrosis in CD ileal resections.
  • These biomarkers show potential for guiding medical and surgical management of refractory CD after further validation.

Keywords:

Related Concept Videos

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy 01:30

314

Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...

Inflammatory Bowel Disease II: Crohn's Disease 01:30

180

Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...

Ultrasound II: Endoscopic Ultrasound and FibroScan 01:25

75

Endoscopic Ultrasound (EUS) and FibroScan are valuable diagnostic tools in gastroenterology and hepatology, each with specific applications and techniques.
Endoscopic Ultrasound (EUS):

Technological Integration: EUS is a sophisticated integration of optical endoscopy and ultrasonography. The endoscope visualizes the mucosal surface directly, while the ultrasound component penetrates deeper to visualize organs and structures beyond the gastrointestinal wall.
Image Formation: The ultrasound...

Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF 01:24

114

Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab...

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy 01:26

48

This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...

Magnetic Resonance Imaging 01:24

4.9K

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...