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Aurora B controls microtubule stability to regulate abscission dynamics in stem cells
Snježana Kodba1, Amber Öztop1, Eri van Berkum1
1Cell Biology, Neurobiology, and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Padualaan, 3584 CS Utrecht, the Netherlands.
Cell Reports
|January 24, 2025
Summary
Microtubules, not passive, control cell division speed by influencing abscission dynamics. High Aurora B activity in stem cells stabilizes microtubules, delaying cell separation.
Area of Science:
- Cell Biology
- Molecular Biology
- Cell Division
Background:
- Abscission, the final stage of cell division, involves severing the cytoplasmic bridge and microtubules.
- The ESCRT machinery mediates abscission, but the role of microtubules in regulating its speed is unclear.
Purpose of the Study:
- To investigate the molecular mechanisms regulating abscission dynamics.
- To identify the role of microtubules in controlling the speed of abscission.
Main Methods:
- Utilized mouse embryonic stem cells undergoing exit from naive pluripotency.
- Analyzed the interplay between Aurora B kinase activity and microtubule stability during abscission.
- Investigated the recruitment of MCAK (Mitotic Centromere-Associated Kinase) to the midbody.
Main Results:
- Demonstrated that microtubules actively control abscission speed.
- Identified a crosstalk between Aurora B activity and microtubule stability.
- Showed that high Aurora B activity in naive stem cells stabilizes microtubules, delaying abscission by reducing MCAK recruitment.
Conclusions:
- Aurora B-dependent microtubule stability is a key regulator of abscission dynamics.
- Stable microtubules enhance Aurora B activity, creating a feedback loop that controls cell division timing.
Keywords:
Aurora BCP: Cell biologyCP: Stem cell researchMCAKabscissioncytoplasmic bridgesmicrotubulesstem cellsMore Related Videos
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