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Related Experiment Video

Updated: May 3, 2026

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
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Basophil activation test; User's manual.

Soren Ulrik Sonder1, Matthew Plassmeyer1, Nikhila Schroeder2

  • 1Amerimmune LLC, 8260 Greensboro Dr, McLean VA-22102, USA.

Journal of Immunological Methods
|January 24, 2025
PubMed
Summary
This summary is machine-generated.

The Basophil Activation Test (BAT) is a key diagnostic tool for allergies, measuring basophil response to allergens. This functional assay aids in allergy diagnosis, monitoring, and guiding treatment decisions.

Keywords:
Allergy testingBATBasophil activation testFlow cytometry

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Area of Science:

  • Immunology
  • Allergy Diagnostics
  • Flow Cytometry

Background:

  • Immediate allergic reactions involve basophils and mast cells, crucial in severe responses.
  • The Basophil Activation Test (BAT) is a flow cytometry assay using CD63 and CD203c to assess allergic reactions.
  • BAT provides a functional readout of patient allergies, measuring concentration-dependent responses to allergens.

Purpose of the Study:

  • To detail the analytical and clinical validation of the Basophil Activation Test (BAT).
  • To review assay validation against regulatory standards (CAP, CLIA, NYS, CLSI).
  • To discuss the utility, interpretation, and limitations of BAT in allergy diagnosis and monitoring.

Main Methods:

  • Flow cytometry analysis of basophil activation markers (CD63, CD203c).
  • Assessment of assay parameters: accuracy, precision, linearity, reportable range, reference range, sensitivity, specificity.
  • Evaluation of pre-analytical considerations and clinical utility.

Main Results:

  • BAT is clinically utilized in the US and globally for allergy diagnosis and monitoring, particularly for food allergens.
  • The article covers comprehensive validation aspects, including regulatory compliance.
  • BAT is essential for patient selection for food challenges and immunotherapies.

Conclusions:

  • BAT is a crucial functional assay for allergy diagnosis and monitoring.
  • The test supports patient management for food challenges and therapies like immunotherapy.
  • Further research and regulatory oversight are needed for optimal clinical integration.