Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity chromatography: a precise method for glycosylated albumin estimation.

W G John, A E Jones

    Annals of Clinical Biochemistry
    |January 1, 1985
    PubMed
    Summary

    This study introduces a precise method for measuring glycosylated albumin in plasma using affinity chromatography. The new technique accurately distinguishes diabetic patients from non-diabetics based on elevated glycosylated albumin levels.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Combined effects of matrix stiffness and obesity-associated signaling directs progressive phenotype in PANC-1 pancreatic cancer cells <i>in vitro</i>.

    bioRxiv : the preprint server for biology·2024
    Same author

    Rising methane: is there a methane emergency?

    Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2021
    Same author

    Determinants of diagnostic discordance for non-diabetic hyperglycaemia and Type 2 diabetes using paired glycated haemoglobin measurements in a large English primary care population: cross-sectional study.

    Diabetic medicine : a journal of the British Diabetic Association·2019
    Same author

    The contact structure of Great Britain's salmon and trout aquaculture industry.

    Epidemics·2019
    Same author

    The UK's suitability for Aedes albopictus in current and future climates.

    Journal of the Royal Society, Interface·2019
    Same author

    Stent angioplasty for treatment of canine valvular pulmonic stenosis.

    Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2019

    Area of Science:

    • Clinical Chemistry
    • Biochemical Analysis
    • Chromatography

    Background:

    • Glycosylated albumin is a biomarker for glycemic control.
    • Accurate measurement of glycosylated albumin is crucial for diabetes management.

    Purpose of the Study:

    • To develop and validate a reliable method for quantifying glycosylated albumin in plasma.
    • To establish a reference range for glycosylated albumin.
    • To compare glycosylated albumin levels in diabetic and non-diabetic individuals.

    Main Methods:

    • Plasma samples were analyzed using affinity chromatography with Glycogel B.
    • Albumin concentration was determined by rocket immunoelectrophoresis.
    • Correlation with glycosylated haemoglobin was assessed.

    Related Experiment Videos

    Main Results:

    • The method demonstrated good precision (CVs 2.8-5.4%) and was unaffected by haemolysis.
    • A reference range of 2.0-5.4% for glycosylated albumin was established.
    • Diabetic patients showed significantly higher glycosylated albumin levels (10.06%) compared to non-diabetics (3.72%).

    Conclusions:

    • Affinity chromatography provides a precise and reliable method for estimating glycosylated albumin.
    • Elevated glycosylated albumin levels are indicative of diabetes.
    • Column loading capacity must not exceed 3 mg albumin per mL of affinity material.