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Lanthanide-Controlled Protein Switches: Development and In Vitro and In Vivo Applications.

Zhong Guo1,2,3, Oleh Smutok4, Chantal Ronacher5

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Summary
This summary is machine-generated.

Researchers engineered novel protein switches for lanthanide detection and extraction. These lanthanide-controlled enzymes offer a new platform for biosensing and microbial engineering applications.

Keywords:
lanthanidesprotein allosteryprotein biosensorsprotein engineeringrare earth elementssynthetic biology

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Area of Science:

  • Biotechnology
  • Biochemistry
  • Materials Science

Background:

  • Lanthanides are crucial rare earth elements with increasing demand in electronics, medicine, and energy storage.
  • Current extraction methods struggle to meet this growing demand.
  • Lanmodulin, a bacterial protein, has shown potential for biotechnological lanthanide detection and extraction.

Purpose of the Study:

  • To engineer novel lanthanide-controlled enzymatic switches.
  • To develop a platform for enhanced lanthanide detection and extraction using engineered proteins.

Main Methods:

  • Construction of β-lactamase-lanmodulin chimeras.
  • Optimization of the chimeric protein switches.
  • Quantification of lanthanide ions using colorimetric and electrochemical assays.
  • Testing E.coli cell growth in the presence of lanthanide ions and β-lactam antibiotics.

Main Results:

  • Engineered switches demonstrated dynamic ranges up to 3000-fold.
  • Accurate quantification of lanthanide ions in simple assays.
  • E.coli cells expressing the chimeras showed lanthanide-dependent growth on β-lactam antibiotics.

Conclusions:

  • Lanthanide-controlled protein switches offer a novel platform for biosensing.
  • This technology enables new approaches for microbial engineering and metal-binding protein design.