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  11. Phylogenetic Analysis And Molecular Structure Of Ns1 Proteins Of Porcine Parvovirus 5 Isolates From Mexico

Phylogenetic analysis and molecular structure of NS1 proteins of porcine parvovirus 5 isolates from Mexico

Alejandro Vargas-Ruiz1, Diana Michele Araiza-Hernández1, Francisco Rodolfo González-Díaz2

  • 1Facultad de Estudios Superiores Cuautitlán, Departamento de Ciencias Biológicas, Universidad Nacional Autónoma de México (UNAM), Carretera Cuautitlán-Teoloyucan Km 2.5, Cuautitlán Izcalli, 54714, Estado de México, México.

Archives of Virology
|January 24, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Porcine parvovirus 5 (PPV5) non-structural protein 1 (NS1) was phylogenetically characterized. Its NS1 protein structure suggests it belongs to the Copiparvovirus genus, offering insights for antiviral drug development.

Area of Science:

  • Virology
  • Molecular Biology
  • Structural Biology

Background:

  • Porcine parvovirus 5 (PPV5) is an unclassified Parvoviridae member.
  • PPV5 is linked to swine diseases but lacks reported pathogenicity.
  • The PPV5 non-structural protein 1 (NS1) is crucial for viral replication and possesses predicted helicase activity.

Purpose of the Study:

  • To conduct phylogenetic and molecular characterization of the PPV5 NS1 protein.
  • To analyze the evolutionary relationships and structural features of PPV5 NS1.
  • To investigate the potential classification of PPV5 within the Parvoviridae family.

Main Methods:

  • Nested PCR and sequencing of PPV5-positive samples from Mexico.
  • Phylogenetic tree construction and sequence identity matrix analysis (nucleotide and amino acid).

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  • Three-dimensional modeling of the PPV5 NS1 protein.

    • Main Results:

    • Sequences representing 96.9% of PPV5 ORF1 were obtained and analyzed.
    • PPV5 NS1 showed highest nucleotide similarity to PPV4 and amino acid similarity to PPV4 and PPV6.
    • The 3D model revealed a C-motif characteristic of Superfamily 3 (SF3) helicases.

    Conclusions:

    • Phylogenetic analysis places PPV5 in close proximity to PPV4 and PPV6.
    • These findings suggest PPV5 may belong to the genus Copiparvovirus.
    • Further research on viral helicases could aid in developing antiviral therapies for human and veterinary medicine.