MOMAST^® Downregulates AQP3 Expression and Function in Human Colon Cells.

Area of Science:

• Cell Biology
• Molecular Biology
• Gastroenterology

Background:

• Aquaglyceroporin 3 (AQP3) facilitates water, glycerol, and hydrogen peroxide transport in human colon cells.
• Aberrant AQP3 expression is linked to diseases with abnormal cell growth and proliferation.
• MOMAST®, a patented antioxidant from olive wastewater, is investigated for its effects on colon cells.

Purpose of the Study:

• To evaluate the effects of MOMAST® on human colon HCT8 cells.
• To investigate MOMAST®'s impact on AQP3 expression and function.
• To explore MOMAST®'s potential as an adjuvant therapy for colon diseases.

Main Methods:

• Cell viability assays were performed on HCT8 cells treated with MOMAST®.
• Confocal microscopy and Western Blotting were used to assess AQP3 expression.
• Functional studies measured glycerol and H2O2 uptake, alongside epithelial-mesenchymal transition (EMT) markers.

Main Results:

• MOMAST® treatment reduced HCT8 cell viability.
• Significant downregulation of AQP3 expression and protein levels was observed.
• MOMAST® decreased glycerol and H2O2 uptake, and reduced levels of EMT markers like vimentin and β-catenin.

Conclusions:

• MOMAST® effectively reduces colon cell viability and AQP3-mediated transport.
• The observed decrease in AQP3 correlates with reduced glycerol and H2O2 uptake.
• MOMAST® shows promise as an adjuvant therapy for colon diseases characterized by abnormal cell growth, by targeting AQP3.