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Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
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EIF4A3-Mediated circ_0008126 Inhibits the Progression and Metastasis of Gastric Cancer by Modulating the

Zeen Wang1, Wenxing Chen1, Ziwei Wang1

  • 1Gastrointestinal Surgical Unit, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Cancers
|January 25, 2025
PubMed
Abstract

Insights

Circular RNA circ_0008126 is downregulated in gastric cancer (GC) and inhibits tumor progression. EIF4A3-mediated circ_0008126 regulates APC expression and inactivates the beta-catenin pathway, suppressing GC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized as key regulators in tumor progression.
  • The specific role and underlying mechanisms of circ_0008126 in gastric cancer (GC) remain largely unexplored.

Purpose of the Study:

  • To investigate the expression levels and clinical significance of circ_0008126 in GC.
  • To elucidate the biological functions and molecular mechanisms of circ_0008126 in GC progression.

Main Methods:

  • In vitro and in vivo experiments were conducted to assess GC phenotypes.
  • Mechanisms involving miR-502-5p, EIF4A3, and APC were explored using RNA pull-down, dual-luciferase reporter assays, RIP, and RNA FISH.

Main Results:

  • Circ_0008126 expression was significantly downregulated in GC tissues and cells, correlating with advanced stage and poor prognosis.
  • Circ_0008126 suppressed GC cell proliferation, metastasis, and epithelial-mesenchymal transition (EMT).
  • EIF4A3 mediates circ_0008126 formation; circ_0008126 binds miR-502-5p to regulate APC expression and inactivate the beta-catenin pathway, enhancing APC mRNA stability.

Conclusions:

  • EIF4A3-mediated circ_0008126 inhibits gastric cancer tumorigenesis and development.
  • Circ_0008126 acts by regulating APC expression and inactivating the beta-catenin pathway via interactions with miR-502-5p and EIF4A3.
Keywords:
APCEIF4A3circ_0008126gastric cancermiR-502-5p

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