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Immunological and biochemical characterization of human adenoviruses from subgenus B. I. Antigenic relationships
Abstract:
47 adenovirus strains related to Ad11, 14, 16, 21, 34, and 35 (including all subgenus B prototypes) were studied in neutralization and hemagglutination-inhibition with 40 antisera, prepared against 16 virus strains, to determine their antigenic relatedness. Wild strains of Ad11-14 (14), Ad14, and a group of 16 strains related to Ad21 proved to be identical within their respective groups, whereas strains related to Ad11, 34, and 35 showed serological differences. Prototype strains of Ad11, 21, 34, and 35 fit the species definition (22) if selected antisera of known specificity are used. All virus strains with Ad16 hemagglutinin showed a bilaterial cross-neutralization either with Ad4, Ad14, Ad3, or Ad21. Doubly neutralizable strains with one hemagglutinin were Ad11 + 35/H11, Ad3 + 16/H16, Ad21 + 16/H16 (H stands for 'hemagglutinin'). Preliminary identification of subgenus B viruses (others than Ad3 and 7) may be performed by HI.
Insights
This study analyzed adenovirus strains using neutralization and hemagglutination-inhibition assays. Adenovirus subgenus B prototypes were classified, revealing distinct antigenic relationships among strains.
Area of Science:
- Virology
- Immunology
Background:
- Adenoviruses, particularly subgenus B, exhibit diverse antigenic properties.
- Accurate classification of adenovirus strains is crucial for understanding viral epidemiology and developing diagnostics.
Purpose of the Study:
- To determine the antigenic relatedness of 47 adenovirus strains, including subgenus B prototypes.
- To evaluate the utility of neutralization and hemagglutination-inhibition assays for adenovirus strain identification.
Main Methods:
- Serological analysis of 47 adenovirus strains using neutralization and hemagglutination-inhibition (HI) assays.
- Testing against 40 antisera prepared from 16 different virus strains.
- Comparison of wild strains and prototype strains for antigenic characterization.
Main Results:
- Wild strains of Ad11-14, Ad14, and Ad21 were found to be antigenically identical within their respective groups.
- Strains related to Ad11, 34, and 35 displayed serological differences.
- Prototype strains of Ad11, 21, 34, and 35 met species definition criteria with specific antisera.
- Adenovirus strains with Ad16 hemagglutinin showed cross-neutralization with Ad4, Ad14, Ad3, or Ad21.
- Doubly neutralizable strains were identified, such as Ad11 + 35/H11.
Conclusions:
- Adenovirus subgenus B strains exhibit distinct antigenic profiles.
- Hemagglutination-inhibition (HI) assays can aid in the preliminary identification of subgenus B viruses (excluding Ad3 and Ad7).
- Specific antisera are essential for accurate adenovirus strain classification and species definition.