Transcriptional Analysis of Tissues in Tartary Buckwheat Seedlings Under IAA Stimulation

Background:Fagopyrum tataricum, commonly referred to as tartary buckwheat, is a cultivated medicinal and edible crop renowned for its economic and nutritional significance. Following the publication of the buckwheat genome, research on its functional genomics across various growth environments has gradually begun. Auxin plays a crucial role in many life processes. Analyzing the expression changes in tartary buckwheat after IAA treatment is of great significance for understanding its growth and environmental adaptability. Methods: This study investigated the changes in auxin response during the buckwheat seedling stage through high-throughput transcriptome sequencing and the identification and annotation of differentially expressed genes (DEGs) across three treatment stages. Results: After IAA treatment, there are 3355 DEGs in leaves and 3974 DEGs in roots identified. These DEGs are significantly enriched in plant hormone signaling, MAPK signaling pathways, phenylpropanoid biosynthesis, and flavonoid biosynthesis pathways. This result suggests a notable correlation between these tissues in buckwheat and their response to IAA, albeit with significant differences in response patterns. Additionally, the identification of tissue-specific expression genes in leaves and other tissues revealed distinct tissue variations. Conclusions: Following IAA treatment, an increase in tissue-specific expression genes observed, indicating that IAA significantly regulates the growth of buckwheat tissues. This study also validated certain genes, particularly those in plant hormone signaling pathways, providing a foundational dataset for the further analysis of buckwheat growth and tissue development and laying the groundwork for understanding buckwheat growth and development.