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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.

Nino-Guy Cassuto1, Florence Boitrelle2, Hakima Mouik3

  • 1ART Unit, Drouot Laboratory, 75009 Paris, France.

Genes
|January 25, 2025
PubMed
Summary

Sperm quality assessment can be improved using microRNA (miRNA) profiling. This study identified specific miRNAs linked to sperm phenotype, offering potential biomarkers for genetic quality evaluation.

Keywords:
biomarkershigh magnificationmale infertilitymicroRNAspermatozoa

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Area of Science:

  • Reproductive Biology
  • Genomics
  • Molecular Biology

Background:

  • Sperm quality is assessed by phenotype, but this doesn't reflect genetic quality.
  • MicroRNAs (miRNAs) play crucial roles in biological processes.
  • Identifying genetic markers for sperm quality is essential.

Purpose of the Study:

  • To identify microRNAs (miRNAs) associated with sperm phenotype.
  • To explore the potential of miRNA profiling as a biomarker for sperm genetic quality.

Main Methods:

  • Analysis of microRNA expression using GeneChip miRNA arrays.
  • Comparison of miRNA profiles in ten semen samples with distinct morphological scores (score 6 vs. score 0).

Main Results:

  • 86 upregulated and 21 downregulated miRNAs were identified in high-quality sperm compared to low-quality sperm.
  • Key upregulated miRNAs include miR-34, miR-30, miR-122, miR-20, miR-182, and miR-191.
  • Predicted targets of upregulated miRNAs are involved in crucial sperm functions like survival, motility, and oxidative stress response.

Conclusions:

  • MicroRNA profiling demonstrates potential as a novel biomarker for assessing sperm quality.
  • This approach could enhance the evaluation of male fertility.