Related Experiment Video
Updated: Jun 18, 2026
Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
Published on: December 22, 2010
Hyaluronan-Mediated Motility Receptor (HMMR) Overexpression Is Correlated with Poor Survival in Patients with B-ALL
Josselen Carina Ramírez-Chiquito1,2, Vanessa Villegas-Ruíz1, Isabel Medina-Vera3
1Experimental Oncology Laboratory, National Institute of Pediatrics, Mexico City 04530, Mexico.
Abstract:
Acute lymphoblastic leukemia (ALL) is a malignant neoplasm with the highest incidence in the pediatric population. Although the 5-year overall survival is greater than 85%, in emerging countries such as Mexico, the mortality rate is high. In Mexico, B-ALL is the most common type of childhood cancer; different characteristics suggest the presence of the disease; however, the prognosis is dependent on clinical and laboratory features, and no adverse prognostic molecular marker for B-ALL has yet been identified. The present research aimed to identify the prognostic value of HMMR expression in pediatric patients with B-ALL. The differential expression profile of B-ALL cells was determined via in silico analysis, and HMMR expression was subsequently measured via qRT-PCR and immunocytochemistry. The results were statistically analyzed via the ROUT test, Kolmogorov-Smirnov Z test, and Mann-Whitney U test. ROC curves and the Youden index were constructed, and Kaplan-Meier curves were plotted. We found that HMMR expression was increased in B-ALL patients (p < 0.0001). We observed that high expression was related to poor prognosis (p < 0.05). We observed that high expression was related to poor prognosis (p < 0.05). The increase in HMMR expression could be a potential early molecular prognostic marker and/or a new target in childhood B-ALL patients.
Insights
Increased HMMR gene expression in childhood B-cell acute lymphoblastic leukemia (B-ALL) is linked to a poorer prognosis. This finding may lead to new early diagnostic markers for pediatric B-ALL patients.
Area of Science:
- Oncology
- Molecular Biology
- Pediatric Hematology
Background:
- Acute lymphoblastic leukemia (ALL) is a prevalent childhood cancer, with higher mortality rates in emerging countries.
- Identifying prognostic markers for B-cell ALL (B-ALL) is crucial for improving patient outcomes, especially in regions like Mexico.
- Currently, no specific adverse molecular prognostic marker for pediatric B-ALL has been established.
Purpose of the Study:
- To investigate the prognostic value of HMMR gene expression in pediatric patients diagnosed with B-ALL.
- To determine if HMMR expression levels can serve as an early indicator of prognosis in childhood B-ALL.
Main Methods:
- In silico analysis to identify differential gene expression profiles in B-ALL cells.
- Quantitative real-time PCR (qRT-PCR) and immunocytochemistry to measure HMMR expression.
- Statistical analysis including ROUT test, Kolmogorov-Smirnov Z test, Mann-Whitney U test, ROC curve analysis, and Kaplan-Meier survival analysis.
Main Results:
- HMMR gene expression was significantly elevated in pediatric B-ALL patients compared to controls (p < 0.0001).
- Higher HMMR expression levels were statistically associated with a poorer prognosis in these patients (p < 0.05).
Conclusions:
- Elevated HMMR expression is a potential early molecular prognostic marker for childhood B-ALL.
- HMMR may represent a novel therapeutic target for improving treatment strategies in pediatric B-ALL.
More Related Videos
08:32Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
Published on: October 25, 2018
06:54Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
Published on: June 15, 2019
