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Ultra-Sensitive Aptamer-Based Diagnostic Systems for Rapid Detection of All SARS-CoV-2 Variants
Sang Won Kim1, Min Jung Han1, Md Shafiqur Rahman1
1Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang-si 37673, Republic of Korea.
International Journal of Molecular Sciences
|January 25, 2025
Summary
New universal aptamer probes detect all SARS-CoV-2 variants, including Omicron. This breakthrough enables rapid and accurate COVID-19 diagnosis using advanced lateral flow and PCR assays.
Area of Science:
- Biotechnology
- Molecular Biology
- Infectious Disease Diagnostics
Background:
- SARS-CoV-2 variants pose diagnostic challenges due to mutations.
- Accurate and rapid COVID-19 detection is crucial for public health.
Purpose of the Study:
- To develop universal aptamer probes for detecting diverse SARS-CoV-2 variants.
- To create sensitive diagnostic platforms for reliable COVID-19 detection.
Main Methods:
- Protein-based and virus-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) were used to identify aptamers.
- Universal aptamer probes were developed targeting conserved regions of SARS-CoV-2 spike proteins.
- A lateral flow assay (LFA) and a rapid PCR technique were integrated for detection.
Main Results:
- Universal aptamer probes demonstrated binding to spike proteins of key SARS-CoV-2 variants, including D614G, Delta, and Omicron.
- A highly sensitive LFA was developed for rapid COVID-19 variant detection.
- An ultra-sensitive molecular diagnostic platform using rapid PCR achieved fast and reliable detection across variants.
Conclusions:
- Universal aptamer probes offer a promising strategy for broad-spectrum SARS-CoV-2 detection.
- The developed diagnostic platforms provide accurate and efficient tools for combating COVID-19.
- This approach addresses the challenge of emerging SARS-CoV-2 variants in diagnostics.
