Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HLA-B*15:01 and HLA-DRB1*07:01 as novel markers of acute GVHD in pediatric HSCT.

Bone marrow transplantation·2026
Same author

Adeno-associated viral vector purification using a centrifugal microfluidic system: towards workflow automation for low-volume sample processing.

Lab on a chip·2026
Same author

Cell-Free DNA Uncovered Tumor Heterogeneity and Informed Targeted Therapy in a Pediatric Rhabdomyosarcoma.

JCO precision oncology·2026
Same author

Thermoplastic elastomer based microfluidic gradient generator for cell culture and drug testing.

Biomedical microdevices·2026
Same author

<i>In situ</i>printing of biphasic jammed inks for conformal deposition on convex anatomical surfaces, with microgravity validation.

Biofabrication·2026
Same author

Deterministic droplet-based co-encapsulation of single cells through inertial and hydrodynamic focusing.

The Analyst·2026

Related Experiment Video

Updated: May 31, 2025

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.0K

Microfluidic Integration of Magnetically Functionalized Microwires for Flow Cytometry Protein Quantification.

Liviu Clime1, Catalin Pavel2, Lidija Malic1

  • 1Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada.

Materials (Basel, Switzerland)
|January 25, 2025
PubMed
Summary

This study introduces a new magnetic bead-based microfluidic system for precise protein quantification. The platform enables automated, sensitive detection of proteins like ICAM-1 in blood plasma for potential point-of-care diagnostics.

Keywords:
flow cytometrymagnetic microwiresmagnetic nanoparticlesmicrofluidicsnanocrystalline magnetsprotein quantification

More Related Videos

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.2K
A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
11:42

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Published on: January 28, 2018

8.6K

Related Experiment Videos

Last Updated: May 31, 2025

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.0K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.2K
A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
11:42

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Published on: January 28, 2018

8.6K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Protein quantification is crucial for diagnostics.
  • Existing methods can be complex and time-consuming.
  • Microfluidic platforms offer miniaturization and automation potential.

Purpose of the Study:

  • To develop a novel microfluidic platform for sensitive protein quantification.
  • To utilize magnetic bead assembly and miniature flow cytometry for analysis.
  • To demonstrate the platform's feasibility for clinical sample analysis.

Main Methods:

  • Functionalized magnetic beads were immobilized on magnetic microwires within microfluidic channels.
  • A fluorescent immunomagnetic sandwich ELISA was performed on-chip.
  • Proteins were quantified by measuring fluorescence intensity using a microfluidic flow cytometer after magnetic bead release.

Main Results:

  • The platform successfully quantified ICAM-1 protein in human blood plasma samples.
  • Performance benchmarking confirmed the feasibility and sensitivity of the approach.
  • The system demonstrated automation and multiplexing capabilities.

Conclusions:

  • The developed microfluidic platform offers a novel and efficient method for protein quantification.
  • The integration of magnetic beads and miniature flow cytometry shows promise for point-of-care applications.
  • This technology has the potential to advance rapid and sensitive diagnostic tools.