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Updated: May 31, 2025

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
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Helicobacter pylori HP0135 Is a Small Lipoprotein That Has a Role in Outer Membrane Stability

Doreen Nguyen1, Rachel G Ivester2, Kyle Rosinke1

  • 1Department of Microbiology, University of Georgia, Athens, GA 30602, USA.

Molecules (Basel, Switzerland)
|January 25, 2025
PubMed

Insights

Helicobacter pylori protein HP0135 is crucial for maintaining cell envelope stability and proper cell division. Deleting this protein causes significant morphological defects in the bacterium.

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Pathogenesis

Background:

  • Helicobacter pylori is a Gram-negative bacterium linked to gastric diseases.
  • The H. pylori flagellum has a sheath derived from the outer membrane.

Purpose of the Study:

  • To investigate the function of the HP0135 lipoprotein in H. pylori.
  • To determine the role of HP0135 in bacterial morphology and cell division.

Main Methods:

  • Proteomic analysis of isolated H. pylori flagella.
  • Genetic deletion of the hp0135 gene in H. pylori B128.
  • Complementation of the hp0135 deletion mutant.

Main Results:

  • HP0135 was identified as a component of the flagellar sheath.
  • Deletion of hp0135 led to outer membrane vesicle formation and mini-cell production.
  • Complementation restored normal morphology to the mutant.

Conclusions:

  • HP0135 plays a vital role in stabilizing the H. pylori cell envelope.
  • HP0135 is essential for normal cell division in H. pylori.
Keywords:
Helicobacter pylorilipoproteinouter membranesmall protein

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