Hormonal and Glycemic Responses During and After Constant- and Alternating-Intensity Exercise

Background/Objectives: Glucose metabolism and hormonal responses are largely affected by exercise intensity, which exhibits an alternating pattern in many sports activities. The aim of this study was to investigate and compare glycemic and hormonal responses during and after exercise of constant (CON) and alternating (ALT) intensity with the same duration and total work. Methods: Ten healthy male volunteers performed two, 60 min cycling exercise bouts in random order: the ALT bout, where exercise intensity alternated between 46.5 ± 1.9% of VO₂max for 40 s and 120% of VO₂max for 20 s, so the mean intensity was at 105% of the lactate threshold (LT), and the CON exercise bout, where the intensity was constant at 105% of LT (70 ± 4.7% of VO₂max). Results: No significant differences were observed in blood glucose concentrations between the two exercise protocols (p = 0.22) or over time at any time point measured, i.e., before, at 30 and 60 min of each exercise bout, and 60 min post-exercise (p > 0.05). Circulating insulin levels decreased significantly over time in both protocols (p < 0.01 and p < 0.05 in ALT and CON, respectively); nevertheless, they did not differ between the exercise protocols (p = 0.45). Similarly, there were no significant differences in serum leptin and prolactin levels between the two protocols (p = 0.77 and p = 0.80 in ALT and CON, respectively); however, circulating levels of leptin decreased at 30 and 60 min of exercise only in CON (p < 0.05) and those of prolactin at 60 min of exercise only in ALT (p < 0.05) compared to pre-exercise values. Conclusions: Cycling exercise of constant or alternating moderate intensity (~70% of VO₂max) with the same duration induces similar glycemic but differential over time hormonal responses in healthy males.