Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

732
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
732
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

643
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
643

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Studies of Foxo1 over the Past 25 Years: Mechanisms of Insulin Resistance and Glucose Dysregulation.

Cells·2026
Same author

eEF2K promotes immune evasion in melanoma via Cyclin D1-mediated stabilization of PD-L1.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Orientin Alleviates Oxidative Stress And Apoptosis In Diabetic Cardiomyopathy Via The Lncrna H19/Mir-103-3p/ALDH2/PI3K/AKT Axis.

Arquivos brasileiros de cardiologia·2025
Same author

Calycosin suppresses the activating effect of granulocyte-macrophage-colony-stimulating factor-producing T helper cells on macrophages in experimental atherosclerosis.

Frontiers in pharmacology·2025
Same author

Bacterial Therapeutics: Addressing the Affordability Gap in Cancer Therapy.

Cancer research·2025
Same author

Cabozantinib selectively induces proteasomal degradation of p53 somatic mutant Y220C and impedes tumor growth.

The Journal of biological chemistry·2025

Related Experiment Video

Updated: May 30, 2025

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

9.8K

eEF-2K Deficiency Boosts the Virus-Specific Effector CD8+ T Cell Responses During Viral Infection.

Liqing Wang1, Benny Shone Song1, Rayansh Poojary1

  • 1Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USA.

Viruses
|January 25, 2025
PubMed
Summary
This summary is machine-generated.

Removing eukaryotic elongation factor-2 kinase (eEF-2K) boosts antiviral CD8+ T cell responses. This enhances effector function and viral clearance without impacting memory cell development, suggesting eEF-2K as a therapeutic target.

Keywords:
T cell immunityTRAF3eEF-2Keffector CD8+ T cellsvaccinia virus (VACV)viral infection

More Related Videos

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
08:52

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

17.1K
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

8.6K

Related Experiment Videos

Last Updated: May 30, 2025

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

9.8K
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
08:52

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

17.1K
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

8.6K

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • T cell responses are crucial for controlling viral infections like vaccinia virus (VACV).
  • Protein synthesis regulation plays a role in T cell function during infection.
  • Eukaryotic elongation factor-2 kinase (eEF-2K) is a known negative regulator of protein synthesis.

Purpose of the Study:

  • To investigate the role of eEF-2K in regulating T cell responses during VACV infection.
  • To determine the impact of eEF-2K deficiency on effector and memory CD8+ T cell populations.
  • To identify mechanisms underlying enhanced antiviral immunity in the absence of eEF-2K.

Main Methods:

  • Utilized eEF-2K-deficient (eEF-2K⁻/⁻) mice and VACV infection model.
  • Analyzed VACV-specific CD8+ T cell populations (effector and memory) via flow cytometry.
  • Performed RNA sequencing on eEF-2K⁻/⁻ VACV-specific effector CD8+ T cells.
  • Investigated the role of TRAF3 in mediating antiviral responses.

Main Results:

  • eEF-2K⁻/⁻ mice showed a higher proportion of VACV-specific effector CD8+ T cells.
  • Memory CD8+ T cell development was not compromised in eEF-2K⁻/⁻ mice.
  • eEF-2K⁻/⁻ effector CD8+ T cells exhibited enhanced functionality and improved viral clearance capacity.
  • Tumor necrosis factor receptor-associated factor 3 (TRAF3) was identified as a key mediator of the enhanced response.

Conclusions:

  • eEF-2K critically regulates T cell responses during viral infections.
  • Targeting eEF-2K can augment effector CD8+ T cell functionality against viral pathogens.
  • This provides a potential novel strategy for enhancing antiviral immunity.