Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Discovery: Overview01:26

Drug Discovery: Overview

7.4K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
7.4K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.4K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.4K
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

6.0K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
6.0K
Drug-Receptor Bonds01:25

Drug-Receptor Bonds

2.7K
Drug-receptor bonds are formed through various chemical forces when drugs interact with target cells. Covalent bonds, strong and irreversible, are exemplified by DNA-alkylating anticancer agents that inhibit cell division. However, such irreversible drug binding lacks selectivity and can modify the DNA of the surrounding healthy cells. Covalent binding often contributes to tissue toxicity, as seen with chloroform and paracetamol metabolites binding to the liver, causing hepatotoxicity.
In...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pulmonary fibrosis after COVID-19 is characterized by airway abnormalities and elevated club cell secretory protein-16.

JCI insight·2026
Same author

Tapt1 deficiency in mice impairs pulmonary lipid homeostasis and normal postnatal respiration by targeting ABCA3 for autophagy-lysosomal degradation.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same author

AIS-based spatiotemporal carbon emission reduction potential of coastal shipping in China.

Journal of environmental management·2026
Same author

Differential associations of lipid profile and genetic susceptibility with sleep duration.

Lipids in health and disease·2026
Same author

An Accurate Genetic Colocalisation Method for the HLA Locus.

HLA·2026
Same author

The illusion of causality: Instruments, colliders, and acute kidney injury in sepsis.

American journal of respiratory and critical care medicine·2026

Related Experiment Video

Updated: May 30, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

4.9K

Caution when using network partners for target identification in drug discovery.

Dandan Tan1, Yiheng Chen2, Yann Ilboudo3

  • 1Quantitative Life Sciences Program, McGill University, MontrĂ©al, QC, Canada; Lady Davis Institute, Jewish General Hospital, McGill University, MontrĂ©al, QC, Canada.

HGG Advances
|January 25, 2025
PubMed
Summary
This summary is machine-generated.

Leveraging human genetic evidence improves drug target identification. However, including protein network partners, while increasing sensitivity, often results in low precision and does not reliably enhance drug target discovery.

Keywords:
IntActStringdbdrug discoverynetwork partnersprotein interaction

More Related Videos

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

2.3K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.6K

Related Experiment Videos

Last Updated: May 30, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

4.9K
Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

2.3K
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

12.6K

Area of Science:

  • Genetics
  • Pharmacology
  • Bioinformatics

Background:

  • Identifying effective drug targets is crucial but challenging, with high failure rates.
  • Human genetic evidence significantly enhances the success rate of drug development.
  • Previous studies suggest ~50% of FDA-approved drugs have genetically validated targets, rising to ~66% when including protein network partners.

Purpose of the Study:

  • To formally test the efficacy of using protein network partners for drug target identification.
  • To assess the impact of including interacting proteins on the precision and sensitivity of genetic target discovery methods.

Main Methods:

  • Utilized the IntAct database to identify physically interacting proteins.
  • Applied methods including exome-wide association studies (ExWASs), genome-wide association studies (GWASs) with the Effector Index, and Genetic Priority Score (GPS).
  • Evaluated the precision, sensitivity, and specificity of identifying positive control genes with and without network partners.

Main Results:

  • Inclusion of molecular interactions increased sensitivity for identifying positive control genes.
  • However, the practical application of network partners was limited by low precision.
  • Expanding genetically identified targets to include network partners did not improve drug target identification across 412 tested traits.

Conclusions:

  • While protein network partners can enhance sensitivity in target identification, their low precision limits practical utility.
  • Expanding genetically identified targets to include network partners should be interpreted with caution due to inconsistent benefits.