Kinetic studies of human erythrocyte membrane resealing

Following lysis in hypotonic media, human erythrocyte membranes will spontaneously reseal and regain their original low permeability for polar solutes. It is generally accepted that resealing will only occur when the membranes are heated above a critical temperature, and that the membrane lesions are stable under cold conditions. Contrary to these prevailing notions, a detailed investigation of the temperature dependence of resealing kinetics over the temperature range 0-22 degrees C revealed that resealing occurs at measurable rates at temperatures as low as 0 degree C, even in buffers of low ionic strength. At all temperatures studied, initial resealing rates were approximately first-order, and Arrhenius plots of these rates revealed a sharp, singular discontinuity at approx. 7 degrees C.