Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to Comment by Quistorff: ATP is not consumed solely by hydrolytic reactions.

Fluids and barriers of the CNS·2025
Same author

Revisiting phenylketonuria: Do high brain glycine levels caused by chronic hyperphenylalanemia contribute to brain dysfunction by modulating D-serine levels and NMDA receptor activity?

Analytical biochemistry·2025
Same author

Comment on the Editorial "Embracing the Modern Biochemistry of Brain Metabolism".

Journal of neurochemistry·2025
Same author

A budget for brain metabolic water production by glucose catabolism during rest, rises in activity and sleep.

Fluids and barriers of the CNS·2025
Same author

Reduced removal of waste products from energy metabolism takes center stage in human brain aging.

Scientific reports·2025
Same author

A functional account of stimulation-based aerobic glycolysis and its role in interpreting BOLD signal intensity increases in neuroimaging experiments.

Neuroscience and biobehavioral reviews·2023

Related Experiment Video

Updated: May 30, 2025

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

14.7K

Setting standards for brain collection procedures in metabolomic studies.

Gerald A Dienel1,2, Thaddeus S Nowak3

  • 1Department of Neurology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|January 25, 2025
PubMed
Summary

Postmortem shifts in labile metabolites can occur within seconds, affecting metabolomics studies. Standardizing sample preparation is crucial for accurate brain metabolite analysis.

Keywords:
Brainlabile metabolitesmetabolomicspostmortem ischemiatissue harvest

More Related Videos

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

428
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.4K

Related Experiment Videos

Last Updated: May 30, 2025

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

14.7K
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

428
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.4K

Area of Science:

  • Biochemistry
  • Neuroscience
  • Analytical Chemistry

Background:

  • Metabolomics technologies offer reliable measurement of numerous chemical entities in tissue extracts.
  • A critical gap exists in addressing enzyme activity during sample preparation, leading to postmortem metabolite alterations.
  • Labile metabolites in brain tissue are particularly susceptible to rapid, significant postmortem changes.

Purpose of the Study:

  • To highlight the issue of postmortem metabolite shifts in brain tissue analysis.
  • To contrast existing studies and identify shortcomings in sample preparation protocols.
  • To propose standardized approaches for future metabolomics research.

Main Methods:

  • Overview of current metabolomics techniques and their limitations regarding postmortem stability.
  • Comparative analysis of studies with varying sample handling procedures.
  • Discussion of strategies to mitigate enzyme activity and preserve metabolite integrity.

Main Results:

  • Postmortem changes in labile metabolites can occur within seconds, especially in brain tissue.
  • Overlooking enzyme inactivation during sample preparation leads to inaccurate metabolite profiles.
  • Established metabolite signatures of in vivo brain serve as a vital validation benchmark.

Conclusions:

  • Standardized sample preparation protocols that rapidly halt enzyme activity are essential for accurate metabolomics.
  • Validation against in vivo metabolite profiles is critical for ensuring data reliability.
  • Adopting new standards will improve the quality and comparability of metabolomics data.