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Engineering conductive covalent-organic frameworks enable highly sensitive and anti-interference molecularly
Ruilin Haotian1, Ziyu Zhu1, Heao Zhang2
1Key Laboratory of Molecular Medicine and Biotherapy, the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Biosensors & Bioelectronics
|January 25, 2025
Summary
Single conductive covalent organic frameworks (COFs) amplify electrochemical signals for sensitive glutathione detection. This approach offers a promising strategy for developing portable, low-cost biosensors.
Area of Science:
- Materials Science
- Electrochemistry
- Nanotechnology
Background:
- Covalent organic frameworks (COFs) are increasingly used in electrochemical sensing.
- Most COFs are co-modified with nanomaterials, limiting their use as standalone signal amplifiers.
- Aza-fuzed π-conjugated COFs show potential for enhanced electron transport and signal amplification.
Purpose of the Study:
- To optimize conductive aza-fuzed π-conjugated COFs for electrochemical sensing.
- To develop a portable molecularly imprinted electrochemical biosensor using a single pristine COF for glutathione detection.
- To evaluate the performance of the COF-based sensor compared to conventional methods.
Main Methods:
- Synthetic engineering was employed to optimize different conductive aza-fuzed π-conjugated COFs.
- The most conductive COF, COF4, was used to modify a screen-printing carbon electrode.
- A molecularly imprinted electrochemical biosensor was constructed for glutathione detection.
Main Results:
- COF4 exhibited the highest conductivity (240% enhancement over bare electrodes).
- The single COF4-modified sensor demonstrated excellent detection performance and selectivity for thiol interferents.
- The COF-based sensor outperformed conventional strategies involving co-modification with gold nanoparticles.
Conclusions:
- Single conductive aza-fuzed π-conjugated COFs can act as effective electrochemical signal amplifiers.
- This strategy provides a promising route for fabricating low-cost, highly sensitive, and selective electrochemical biosensors.
- The developed biosensor is suitable for point-of-care glutathione detection.
