Isolation, characterization, and genome sequencing analysis of a novel phage HBW-1 of Salmonella

Salmonella presents a significant threat to the health of animals and humans, especially with the rise of strains resistant to multiple drugs. This highlights the necessity for creating sustainable and efficient practical approaches to managing salmonellosis. The most recent and safest approach to combat antimicrobial resistance-associated infections is lytic bacteriophages. This study recovered a Salmonella-specific phage HBW-1 from sewage and faecal samples from commercial chicken farms in Henan, China. Transmission electron microscopy showed that the phage possesses a polyhedral head and a tailed structure characteristic of bacteriophages. The phage HBW-1 exhibited favorable stability when subjected to elevated temperatures between 30 °C and 60 °C and pH levels between 3 and 12 for 1 h. The phage genome consists of double-stranded, circular DNA with a size of 43,095 bp and a GC content of 49.54 %. Notably, phage HBW-1 contains 62 genes encoding proteins and does not contain virulence or resistance genes commonly found in bacteria. Phage spectrum analysis indicates that the phage HBW-1 is strictly a lytic, exhibiting antibacterial activity against Salmonella Pullorum (100 %, n = 11), Salmonella Typhimurium (92.86 %, n = 42) and Salmonella Enteritidis (58.97 %, n = 39). Therefore, this study suggests that phage HBW-1 holds promise as a potential alternative for prevention and control of Pullorum Disease.