Related Experiment Video
Updated: May 30, 2025
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
Published on: April 11, 2013
Colorimetric and fluorescent probe assisted by smartphone app for monitoring fish freshness
Xiaofei Sun1, Xin Jiang2, Xinyi Zhao2
1College of Food Science and Engineering, College of Chemistry and Materials Engineering, Institute of Ocean, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, PR China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, PR China.
Abstract:
In this study, a novel "OFF-ON" fluorescent probe MPZ ((E)-5-((10-ethyl-2-methoxy-10H-phenothiazin-3-yl)methylene)thiazolidine-2,4-dione) based on phenothiazine is synthesized, which can rapidly (7 s) detect biogenic amines (BAs) through deprotonation, utilizing both colorimetric and fluorescent dual channels. An app for visual portable detection of fish freshness, named "Visual Evaluation", is independently developed. This app integrates several functions, including image capture, editable scanning of red, green, and blue (RGB) values, data analysis fitting, data storage, and verification. More importantly, the smart detection platform composed of the app and MPZ-loaded filter paper tags (MPZ/FPS) can quantitatively detect the total volatile basic nitrogen (TVB-N) value in salmon and has been validated for its accuracy and reliability using the national standard method of China. This smart detection platform is expected to provide new ideas for the field of portable visual inspection, and also offer merchants, and regulators a new instrument-free and intelligent reading tool for assessing the freshness of salmon.
