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Updated: May 30, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Chemiluminescence of silver and nitrogen doped carbon dots induced by potassium ferricyanide/hydrogen peroxide and
Yamei Jiang1, Jiaoyan Xie1, Xiaowei Wang1
1School of Chemistry and Material Science, Shanxi Normal University, Taiyuan 030000, Shanxi, PR China.
Abstract:
In this study, carbon dots doped with silver and nitrogen (Ag,N-CDs) were synthesized and their application in chemiluminescence (CL) was investigated using the potassium ferricyanide/hydrogen peroxide (K3Fe(CN)6/H2O2) reaction. Theoretical calculations reveal that Ag doping facilitates a lower excitation energy. The experimental conditions influencing the CL reaction were examined and optimized. The resulting Ag,N-CDs-based CL method demonstrated impressive capabilities in detection of Cu(II) within a linear calibration range of 40.0 nM-8.0 μM, with a commendable detection limit of 3.1 nM. In extensive validation experiments, recoveries and relative standard deviations for water, plasma, and tea samples consistently fell within the range of 97.0-103.0 % and 1.03-4.19 %, respectively. These outcomes underscore that the CL method based Ag,N-CDs is an excellent tool for the detection of Cu(II).
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