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Developing affordable and efficient heating devices for enhanced live cell imaging in confocal microscopy.

Abhishesh Bajracharya1, Sampada Timilsina1, Ruofan Cao2

  • 1Department of Biology, University of Mississippi, University, MS, United States.

Frontiers in Plant Science
|January 27, 2025
PubMed
Summary

Researchers developed two affordable heating devices for live plant cell imaging. These tools enable precise temperature control for studying plant responses to heat stress, making advanced research accessible to more labs.

Keywords:
confocal microscopycost-effectiveheat shock proteinheat stresslive cell imaginglow-maintenancemicroheaterstress granules (SG)

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Area of Science:

  • Plant Biology
  • Microscopy
  • Thermal Biology

Background:

  • Accurate temperature control is essential for live cell imaging, especially when studying plant responses to thermal stress.
  • Existing solutions for temperature control in microscopy can be expensive and may not meet the specific needs of plant research.

Purpose of the Study:

  • To design, develop, and test two cost-effective heating devices for confocal microscopy applications.
  • To provide accessible tools for studying plant thermal biology, including responses to moderate and severe heat stress.

Main Methods:

  • Engineered an aluminum heat plate to fit standard microscope stages, supporting temperatures up to 36°C.
  • Developed a wireless mini-heater for rapid, precise sample slide heating up to 50°C.
  • Tested devices in controlled studies, including real-time analysis of heat shock protein accumulation and stress granule formation in *Arabidopsis thaliana*.

Main Results:

  • The aluminum heat plate effectively maintained temperatures for moderate heat stress studies (30-36°C).
  • The wireless mini-heater demonstrated rapid heating and maintained high temperatures (up to 50°C) for severe heat stress investigations.
  • Both devices proved effective in live imaging experiments and had a combined construction cost under $300.

Conclusions:

  • The developed heating devices are effective, affordable, and accessible solutions for live plant cell imaging under thermal stress.
  • These budget-friendly tools can significantly benefit smaller laboratories with limited funding for specialized equipment.
  • Future improvements could enhance heat uniformity, humidity control, and thermal stability for extended imaging sessions.