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3D scaffold of hydroxyapatite/β tricalcium phosphate from mussel shells: Synthesis, characterization and cytotoxicity
Sabrina Cunha da Fonseca1, Rosangela Borges Freitas1, Anne Raquel Sotiles2
1Department of Cell Biology, Federal University of Paraná, Post box-19031, Zip code -81531-970, Curitiba, PR, Brazil.
Abstract:
Bone tissue substitutes are increasing in importance. Hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) act as a cell matrix and improve its mechanical properties. One of their raw materials is marine-origin by-products.
Objectives:
To synthesize, characterize, and evaluate the cellular cytotoxicity of a 3D biomaterial based on HA and β-TCP from mussel shells.
Methods:
We prepared pellets with 150, 200, and 250 mg and evaluated them through sintering, XRD, FTIR, ICP-OES, Scanning Electron Microscopic (SEM), and immunocytochemical tests. The Alamar Blue method was applied to the Balb-T3T cell line within 72 h to evaluate cytotoxicity.
Results:
Our biomaterials presented a smooth surface with slight irregularity and porosities presenting different diameters and morphologies and showed chemical, morphological, and ultrastructural similarity to bone hydroxyapatite, mainly the 150 and 200 mg pellets.
Significance:
We produced promising HA/β-TCP bioceramics with characteristics that allowed cell culture, promoting adhesion, spreading, and proliferation.
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