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Updated: May 30, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

From isolation to detection, advancing insights into endothelial matrix-bound vesicles.

Sahimy Ayus-Martinez¹, William Meza-Morales¹, Jesus Jimenez-Osorio¹

¹Department of Chemical Engineering, University of Puerto Rico-Mayaguez, Route 108, Mayaguez, Puerto Rico, USA.

Extracellular Vesicle

|January 27, 2025

Summary

Researchers isolated matrix-bound vesicles (MBVs) from blood vessel walls and cartilage. They developed a biosensor to detect CD144+ MBVs, advancing understanding of extracellular matrix signaling for regenerative medicine.

Keywords:

Biomarker Biosensor Electrochemical detection Endothelial extracellular vesicles Matrix-bound vesicles VE-Cadherin-CD144−

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Last Updated: May 30, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

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Published on: February 28, 2025

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

Area of Science:

Biomedical Engineering
Cell Biology
Extracellular Matrix Research

Background:

Matrix-bound vesicles (MBVs) are key components of the extracellular matrix (ECM) involved in molecular signaling.
Understanding MBVs' role in tissue-specific signaling is crucial for biomedical applications.

Purpose of the Study:

To isolate and characterize MBVs from porcine arterial endothelial cell basement membranes (A-MBVs) and thyroid cartilage (C-MBVs).
To develop a novel biosensor for detecting CD144+ MBVs.
To explore the potential of MBVs in regenerative medicine and ECM signaling.

Main Methods:

Isolation of MBVs from A-MBVs and C-MBVs.
Orthogonal characterization using Transmission Electron Microscopy (TEM), Nano-Tracking Analysis (NTA), Electrochemical Impedance Spectroscopy (EIS), and Atomic Force Microscopy (AFM).
Development of an immuno-functionalized screen-printed electrode for CD144+ MBV detection and characterization.

Main Results:

Successful isolation of A-MBVs and C-MBVs, with A-MBVs showing vascular endothelial cadherin (CD144) and C-MBVs showing SOX9.
Development of a functional biosensor capable of immunoadsorbing and electrochemically detecting CD144+ MBVs.
AFM confirmed the integrity and morphology of CD144+ MBVs after immunoadsorption.

Conclusions:

MBVs are crucial conveyors of tissue-specific signals.
The developed biosensor enables efficient detection and characterization of specific MBVs.
This research opens new avenues for utilizing MBVs in regenerative medicine and understanding ECM signaling.