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Updated: May 30, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
AKT activation participates in Fascin-1-induced EMT in hepatoma cells
Pengju Zhao1, Kewei Ai1, Yi Li1
1The Second Department of General Surgery, First Affiliated Hospital of Dali University, Dali, 671000 Yunnan China.
Abstract:
High expression of Fascin-1 involves high metastasis, high recurrence, and poor prognosis of cancers. However, the related regulatory mechanism in hepatocellular carcinoma (HCC) remains elusive. In this study, Fascin-1 was highly expressed in HCC tissues and cell lines. Fastin-1 protein levels and p-Akt1/Akt1 rate were increased by Akt activator SC79 and were decreased by Akt inhibitor LY294002. Silenced Fascin-1 suppressed cell proliferation, promoted cell apoptosis, suppressed cell invasion and epithelial-mesenchymal transition (EMT) in HCC cell lines. Also, silenced Fascin-1 induced cell cycle arrest in the G1 phase. Moreover, silenced Fascin-1 repressed invasion of HCC cells by inhibiting EMT. Besides, interference with Fascin-1 inhibited HCC cell growth, reduced Vimentin expressions and p-Akt1/Akt1 rate in vivo, while these impacts were abolished after injection of SC79. In conclusion, silencing Fascin-1 reduced the malignant growth of HCC, and this process was closely related to AKT inactivation.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10616-025-00707-9.
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