Pan-cancer analysis of Arp2/3 complex subunits: focusing on ARPC1A's role and validating the ARPC1A/c-Myc axis in non-small cell lung cancer
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.The Arp2/3 complex subunit ARPC1A is overexpressed in many cancers, linked to poor prognosis and diagnostic value. Targeting ARPC1A offers a potential anti-cancer strategy and biomarker for diagnosis and therapy response.
Area Of Science
- Oncology
- Molecular Biology
- Cancer Metastasis Research
Background
- The Arp2/3 complex is crucial for tumor metastasis, yet its subunits' clinical relevance is unclear.
- Understanding Arp2/3 complex subunit expression, prognosis, and diagnostic value across cancers is needed.
- This study focuses on the clinical significance of Arp2/3 complex subunits, especially ARPC1A, in pan-cancer analysis.
Purpose Of The Study
- To investigate the pan-cancer clinical relevance of Arp2/3 complex subunits, with a focus on ARPC1A.
- To analyze ARPC1A's biological mechanisms, immune infiltration associations, and chemotherapy drug sensitivity.
- To explore ARPC1A's role in non-small cell lung cancer (NSCLC) progression and potential therapeutic targeting.
Main Methods
- Analyzed TCGA and GTEx data for Arp2/3 subunit expression and clinical relevance.
- Utilized TCPA and TIMER2.0 for ARPC1A-immune infiltration and protein interaction analysis.
- Performed GSEA, CellMiner, GDSC, CTRP analyses, qPCR, Western blot, and <i>in vitro</i> assays (CCK-8, EdU, colony formation, Transwell) for validation.
Main Results
- ARPC1A is frequently overexpressed in cancers, correlating with poor prognosis and showing diagnostic utility.
- Copy number variations may influence Arp2/3 subunit expression; X4.5.dianilinophthalimide shows therapeutic potential.
- ARPC1A is linked to oxidative phosphorylation, MYC-mediated proliferation, chemoresistance (gefitinib), and enhanced NSCLC malignancy via c-Myc.
Conclusions
- Arp2/3 complex subunits, particularly ARPC1A, represent promising anti-cancer targets.
- ARPC1A serves as a potential biomarker for tumor diagnosis, prognosis, and predicting immune therapy response.
- Targeting Arp2/3 complex offers novel anti-cancer strategies and insights into tumor progression mechanisms.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Related Concept Videos
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...