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Transfer of extracellular matrix components between germ layers in chimaeric chicken-quail blastoderms
Cell and Tissue Research
|January 1, 1985
Summary
Chemical signals are transmitted from the hypoblast to the epiblast during gastrulation. Macromolecules are deposited by hypoblast filopodia onto the epiblast basement membrane, guiding embryonic development.
Area of Science:
- Developmental biology
- Cell signaling
- Embryology
Background:
- Gastrulation involves complex cell signaling.
- The hypoblast's role in early embryonic signaling is not fully understood.
Purpose of the Study:
- To investigate the chemical basis of signal transmission during gastrulation.
- To identify the source and mechanism of signaling molecules.
Main Methods:
- Chimeric embryos created from chicken and quail embryos.
- Autoradiography using 3H-glucosamine labeling.
- Feulgen staining for cell origin identification.
- Scanning electron microscopy for morphology.
Main Results:
- Labeled macromolecules transferred from quail hypoblast to chicken epiblast.
- Transfer occurred to the epiblast basement membrane, not between hypoblast cells.
- Hypoblast filopodia directly deposited macromolecules.
- Diffusion of free macromolecules was unlikely.
Conclusions:
- Hypoblast actively transmits signals via deposited macromolecules.
- This process is crucial for gastrulation and embryonic development.
- Epiblast cell intervention is not required for this transfer.