The characteristics of aminotransferases gene family in Ruditapes philippinarum and its response to salinity stresses
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View abstract on PubMed
Summary
This summary is machine-generated.This study identified 28 aminotransferase genes in R. philippinarum, revealing their differential expression under salinity stress. These findings highlight the role of aminotransferases in the salinity adaptation of aquatic animals.
Area Of Science
- * Marine Biology
- * Molecular Biology
- * Biochemistry
Background
- * Aminotransferases regulate amino acid metabolism, crucial for homeostasis and environmental adaptation in aquatic organisms.
- * Maintaining amino acid balance is vital for aquatic animal survival in varying conditions.
Purpose Of The Study
- * To identify and characterize aminotransferase genes in R. philippinarum.
- * To investigate the expression patterns of these genes under different salinity conditions.
- * To understand the role of aminotransferases in the physiological response to salinity stress.
Main Methods
- * Genome-wide identification of 28 aminotransferase genes in R. philippinarum.
- * Bioinformatics analysis including gene structure, protein structure, chromosome localization, and phylogenetic analysis.
- * RNA-sequencing (RNA-seq) and quantitative real-time PCR (qPCR) to analyze gene expression.
- * Measurement of free amino acid content in gills under varying salinity.
Main Results
- * Aminotransferase genes were classified into classes I/II, III, and V based on structural and phylogenetic analyses.
- * Significant differential expression of aminotransferase genes observed across developmental stages, tissues, and salinity levels.
- * Gene expression analysis revealed increased aminotransferase gene activity under salinity changes.
- * Free amino acid content in gills significantly decreased under low salinity and increased under high salinity stress.
Conclusions
- * Aminotransferase genes play a significant role in the salinity adaptation of R. philippinarum.
- * Differential expression and amino acid content changes indicate a regulatory function in response to osmotic stress.
- * Provides a foundation for further research into aminotransferase-mediated salinity tolerance mechanisms.
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