Construction, characterization and application of rutin loaded zein - Carboxymethyl starch sodium nanoparticles

In this paper, zein-carboxymethyl starch (CMS) nanoparticles were prepared by antisolvent precipitation method to improve the stability of rutin (RT). The encapsulation efficiency, loading capacity, oxidation resistance, structural properties were evaluated. The results showed that electrostatic, hydrogen bond and hydrophobic interaction were the main driving forces for the formation of nanoparticles. The size and PDI of all prepared zein-RT-CMS nanoparticles were <190 nm and 0.3, respectively, and the zeta-potential was about -25 mV. When zein /RT/CMS was 1:0.02:3, the maximum encapsulation rate of nanoparticles reached 86.2 %. The constructed stabilized RT significantly improved the antioxidant activity of free RT, and the antioxidant activity of RT was up to 88 %. In addition, a high temperature and low humidity simulation system with asparagine/glucose as the reaction substrate was established to study the inhibitory effect of zein- RT- CMS carrier on the production of acrylamide. The results showed that the zein-CMS carrier could improve the thermal stability of RT and inhibit the formation rate of acrylamide in thermal processing. When zein /RT/CMS was 1:0.02:3, the inhibition rate of acrylamide could reach 33.51 %, thus reducing acrylamide formation in thermal processing.