Research on bioaugmented slurry remediation of PAHs in actual contaminated soil: Screening microbial agents and...

Bioaugmented slurry technology is a sustainable remediation technology for PAHs-contaminated soil. However, the lack of experimental data on the remediation of complex, actual contaminated soils has hindered the practical application of this technology. This study explored the bioaugmented degradation of PAHs using actual soil slurry with and without the addition of microbial agents in the microscopic world. NS4 has the highest degradation efficiency. The response surface method was used to determine the effects of water-soil ratio, temperature, aeration rate and their interaction on the degradation of PAHs. Temperature significantly affects the degradation of phenanthrene, and the aeration rate significantly affects the degradation of pyrene. The influence of each factor follows the order: aeration rate > temperature > water-soil ratio. The highest degradation rates of phenanthrene and pyrene are observed at a water-soil ratio of 3:1, a temperature of 30 °C, and an aeration rate of 2 L/min. Under the optimal conditions, the addition of either peptone or Tween-80 increased the degradation rate. Peptone and Tween-80 can effectively enhance the growth rate of microorganisms and the release of PAHs in actual contaminated soil. In conclusion, by screening microbial agents suitable for real contaminated soils and maintaining the dynamic stability of the bioaugmented slurry system by optimizing key influencing factors, efficient, green, and low-energy remediation of PAHs-contaminated sites can be achieved.