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Toxoplasmosis01:28

Toxoplasmosis

Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...

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Updated: Jul 8, 2026

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Analysis of Seroreactivity and Seropositivity in Balb/c Mice Experimentally Infected with Toxocara canis Using Two

Kaveri Theerthagiri Kavitha1, Chirukandoth Sreekumar2, Bhaskaran Ravi Latha3

  • 1Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India. kavitharavi2004@yahoo.com.

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|January 27, 2025
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Summary

This study found that the rTc-CTL-1 antigen ELISA is superior for diagnosing toxocarosis in mice, detecting antibodies earlier and more effectively than the rTES-120 antigen ELISA. Higher egg doses led to a stronger antibody response, indicating dose-dependent diagnosis for toxocarosis.

Keywords:
Toxocara canisBalb/c miceELISASeroreactivityrTES-120rTc-CTL-1

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Area of Science:

  • Veterinary Parasitology
  • Immunodiagnostics
  • Molecular Biology

Background:

  • Human toxocarosis diagnosis relies on serological assays detecting antibodies to Toxocara antigens.
  • Toxocara canis larvae do not mature in paratenic hosts, making mouse models crucial for understanding human infection.
  • Experimental mouse infections mimic human toxocarosis, aiding in better comprehension of the disease.

Purpose of the Study:

  • To evaluate the diagnostic efficacy of two recombinant Toxocara antigens, rTc-CTL-1 and rTES-120, in an experimental mouse model.
  • To compare the sensitivity and timing of antibody detection using rTc-CTL-1 and rTES-120 based ELISAs.
  • To assess the correlation between infection dose and antibody response in mice.

Main Methods:

  • Two recombinant antigens (rTc-CTL-1, rTES-120) were expressed in E. coli.
  • Balb/c mice were infected with 100 or 1000 T. canis eggs (Groups I and II) or left uninfected (Group III).
  • Serum samples were collected serially and tested via indirect ELISA for antibody responses to Toxocara antigens.

Main Results:

  • rTc-CTL-1 ELISA detected antibodies from 7 days post-infection (dpi), peaking at 28 dpi and persisting up to 150 dpi.
  • rTES-120 ELISA detected antibodies from 28 dpi, peaking at 60 dpi and persisting up to 150 dpi.
  • Seropositivity rates at 28 dpi were 87.5% (Group I) and 100% (Group II) for rTc-CTL-1, and 12.5% (Group I) and 25% (Group II) for rTES-120, with significantly higher responses in Group II.

Conclusions:

  • rTc-CTL-1 antigen ELISA demonstrates superior diagnostic efficacy for toxocarosis compared to rTES-120 ELISA.
  • rTc-CTL-1 facilitates earlier antibody detection, crucial for timely diagnosis of toxocarosis.
  • The antibody response is directly proportional to the infective egg dosage, validating the dose-dependent nature of the diagnostic assay.