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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Related Experiment Video

Updated: May 30, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
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In Situ Analysis of Plant Tissue Using Arc iKnife Ionization Mass Spectrometry.

Wen Cai1,2, Yuanji Gao3, Changsheng Shi4

  • 1Key Laboratory for Biorheological science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, People's Republic of China.

Analytical Chemistry
|January 28, 2025
PubMed
Summary

A novel portable arc iKnife ionization mass spectrometry (AII-MS) technique analyzes plant tissues using plasma. This method offers rapid, reliable, and sustainable analysis of diverse plant samples without charring.

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Area of Science:

  • Analytical Chemistry
  • Plant Science
  • Mass Spectrometry

Background:

  • Traditional plant tissue analysis methods can be time-consuming and may require extensive sample preparation.
  • Arc ionization techniques often face challenges with aerosol formation and tissue charring.

Purpose of the Study:

  • To develop a portable and efficient method for direct plant tissue analysis using arc ionization mass spectrometry.
  • To overcome limitations of existing arc ionization methods, such as charring and aerosol issues.

Main Methods:

  • Integration of a surgical knife with low-temperature arc plasma for direct plant tissue interaction.
  • Utilizing plasma-generated ions to charge sputtered aerosols from plant tissues for mass spectrometry detection.
  • Employing nitrogen cooling and a carrier gas to manage sample integrity and aerosol transfer.

Main Results:

  • The developed arc iKnife ionization mass spectrometry (AII-MS) effectively analyzes various fresh and dried plant tissues (seeds, fruits, leaves, roots, stems, flowers, bark).
  • Characteristic components were identified in both positive and negative ion modes.
  • Potassium nitrate was detected in plant samples, potentially due to its use as a fertilizer.

Conclusions:

  • AII-MS provides a high-throughput, environmentally sustainable, and rapid method for reliable plant tissue analysis.
  • The technique minimizes sample damage and overcomes charring issues associated with arc ionization.
  • This method enables direct analysis of diverse plant materials, offering new insights into their chemical composition.