Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Flora of the Large Intestine

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.

Microbes in Food Production

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...

Microbes in the Production of Fermented Foods

Microbes in the Production of Fermented Foods

Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...

Probiotics

Probiotics

Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Microbiota of the Large Intestine

Microbiota of the Large Intestine

The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting the Energy-Coupling Factor Transporters: A Novel Antibiotic Drug Target in <i><i>Streptococcus pneumoniae</i></i>.

Journal of medicinal chemistry·2026

Same author

Hit-to-Lead Optimization of Energy-Coupling Factor (ECF) Transporter Inhibitors as Novel Antibiotic.

Journal of medicinal chemistry·2026

Same author

Aggregicyclins Shed Light on Type II Polyketide Biosynthesis in <i>Myxococcota</i>.

JACS Au·2026

Same author

Incorporation of imidazolium chitosan derivative yields scaffolds with enhanced antioxidant, antimicrobial and immunomodulatory properties.

Regenerative biomaterials·2026

Same author

Integrating chemical, genetic, and feasibility assessments for anti-tubercular target validation.

EMBO molecular medicine·2026

Same author

Genome Mining Guided Identification of the Metallophore Delftichelin A from <i>Delftia deserti</i>.

Journal of natural products·2026

Search research articles

Related Experiment Video

Updated: May 14, 2026

Generation of Alginate Microspheres for Biomedical Applications

Generation of Alginate Microspheres for Biomedical Applications

Published on: August 12, 2012

PEARL: Protein Eluting Alginate with Recombinant Lactobacilli.

Varun Sai Tadimarri^1,2, Marc Blanch-Asensio^1,2, Ketaki Deshpande^1,2

¹INM - Leibniz Institute for New Materials, Saarland University, Campus D2 2, 66123, Saarbrücken, Germany.

Small (Weinheim an Der Bergstrasse, Germany)

|January 28, 2025

Summary

This summary is machine-generated.

Engineered living materials (ELMs) using probiotic lactobacilli in alginate beads enable sustained protein release for 14 days. This PEARL system offers bacterial containment and stable therapeutic delivery.

Keywords:

alginate engineered living materials lactobacillus secretion synthetic biology

More Related Videos

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

Related Experiment Videos

Last Updated: May 14, 2026

Generation of Alginate Microspheres for Biomedical Applications

Generation of Alginate Microspheres for Biomedical Applications

Published on: August 12, 2012

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Published on: June 29, 2017

Area of Science:

Biotechnology
Materials Science
Microbiology

Background:

Engineered living materials (ELMs) using bacteria in hydrogels show promise for therapeutic applications.
Lactic acid bacteria, particularly lactobacilli, are favored for probiotics due to inherent health benefits.
Limited genetic tools in lactobacilli hinder their use in advanced ELMs.

Purpose of the Study:

To enhance the genetic programmability of Lactiplantibacillus plantarum WCFS1 for protein secretion.
To develop a cost-effective and biocompatible core-shell alginate bead for encapsulating engineered lactobacilli.
To create a novel ELM for controlled and sustained release of recombinant proteins.

Main Methods:

Genetic engineering of Lactiplantibacillus plantarum WCFS1 for enhanced protein secretion.
Encapsulation of engineered bacteria within core-shell alginate beads.
Assessment of protein release profiles and bacterial containment over 14 days.

Main Results:

Successful expansion of genetic programmability in L. plantarum WCFS1 for protein secretion.
Development of PEARL (Protein Eluting Alginate with Recombinant Lactobacilli), a novel ELM.
Demonstrated controlled and sustained release of recombinant proteins for up to 14 days.
Observed benefits of encapsulation, including bacterial containment and stabilized release profiles.

Conclusions:

Recombinant lactobacilli encapsulated in alginate beads offer a viable strategy for therapeutic protein delivery.
The PEARL system provides mutual benefits, enhancing both bacterial function and protein release characteristics.
This approach overcomes limitations in lactobacilli genetic tools for developing advanced ELMs.