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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

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Related Experiment Video

Updated: Jun 25, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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Development of a tissue-engineered skin model with epidermal, dermal and hypodermal components.

V L Workman1, A-V Giblin2, N H Green1

  • 1Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK.

In Vitro Models
|January 28, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel trilayer skin model incorporating the hypodermis, crucial for skin health. This advanced tissue-engineered skin offers a more complete in vitro model for studying skin function and disease.

Keywords:
AdiposeCytokeratinDermal-epidermal junctionHypodermisRete ridgesSkinTissue engineering

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Dermatology

Background:

  • Tissue-engineered skin models are vital for research but often lack a hypodermal layer.
  • The hypodermis plays a critical role in skin homeostasis and disease, yet is understudied due to a lack of appropriate models.

Purpose of the Study:

  • To develop and characterize a physiologically relevant, tissue-engineered trilayer skin model including epidermal, dermal, and hypodermal layers.

Main Methods:

  • Cultured human keratinocytes and fibroblasts on decellularized human dermis combined with explanted human adipose tissue.
  • Compared bilayer (epidermis-dermis) and trilayer (epidermis-dermis-hypodermis) models.

Main Results:

  • The trilayer model demonstrated improved dermal-epidermal junction morphology and rete ridge formation compared to bilayer models.
  • Cell proliferation (Ki-67) and cytokeratin expression (AE1/AE3, cytokeratin 14) were comparable to native skin tissues over 28 days.
  • The model preserved native extracellular matrix architecture and cellular heterogeneity.

Conclusions:

  • A novel, physiologically relevant trilayer tissue-engineered skin model incorporating the hypodermis was successfully developed.
  • This model offers a valuable tool for research requiring a functional hypodermal component, advancing the study of skin homeostasis and diseases.