Related Experiment Video
Updated: May 30, 2025
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
Published on: October 19, 2021
Cell surface protein-protein interaction profiling for biological network analysis and novel target discovery
Jiaojiao Chen1, Maoxin Fang1, Yuwei Li1
1Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
Secretome protein-protein interactions (SPPIs) are vital for cellular communication and biological processes. Understanding SPPIs aids in identifying therapeutic targets for cancer, immune disorders, and infectious diseases.
Area of Science:
- Proteomics
- Cell Biology
- Immunology
Background:
- The secretome, comprising secreted and cell surface proteins, is crucial for intercellular communication.
- Secretome protein-protein interactions (SPPIs) regulate fundamental cellular processes like proliferation and apoptosis.
- Dysregulated SPPIs are implicated in diseases such as cancer, immune disorders, and infections.
Purpose of the Study:
- To review and discuss technologies for secretome protein-protein interaction (SPPI) profiling.
- To highlight the application of SPPI profiling in identifying novel therapeutic targets.
- To address challenges in identifying SPPIs, including orphan receptors/ligands and unknown viral host interactions.
Main Methods:
- Review of various established and emerging technologies for SPPI profiling.
- Analysis of the application of these technologies in basic and translational research.
- Discussion of methods for identifying receptor/ligand pairs within the SPPI network.
Main Results:
- Multiple technologies exist for comprehensive SPPI profiling.
- SPPI profiling has broad applications in identifying novel therapeutic targets.
- Significant challenges remain in characterizing many SPPIs and their functions.
Conclusions:
- SPPI profiling technologies are essential tools for understanding cellular communication and disease mechanisms.
- Identifying SPPIs offers promising avenues for developing novel immunotherapies and anti-infective agents.
- Further research is needed to elucidate the vast number of uncharacterized SPPIs and their roles.
More Related Videos
08:38Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
Published on: March 3, 2015
08:31Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
Related Concept Videos
02:26Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:04Protein-protein Interfaces