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Fluorescent nucleobase analogue for cellular visualisation and regulation of immunostimulatory CpG
Tatum Melati Andini1,2, Satoshi Tada3, Tomotaka Kumagai4
1Immunology Frontier Research Center, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan. spark@ifrec.osaka-u.ac.jp.
Abstract:
In this study, we explored the chemical modification of toll-like receptor 9 (TLR9) agonist DNA using a highly fluorescent thymine analogue, ThexT, focusing on its structural and photophysical characteristics. ThexT-labelled CpG oligonucleotides effectively demonstrated intracellular localisation within macrophage cell lines. Notably, immunostimulatory activity varied depending on the site of ThexT incorporation within the TLR9 agonist sequence. The introduction of fluorescent nucleobases offers a useful approach for visualising immunostimulatory oligonucleotides and for modulating immune responses.
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