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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

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Electrochemical capacitance-based aptasensor for HER2 detection.

Daísy Camargo Ferreira1, Marina Ribeiro Batistuti Sawazaki2, Bassam Bachour Junior1

  • 1Department of Physics, Faculty of Philosophy, Science and Letter, University of São Paulo, Ribeirão Preto, SP, 14040-901, Brazil.

Biomedical Microdevices
|January 28, 2025
PubMed
Summary

We developed a simple, highly sensitive electrochemical platform for detecting Human Epidermal Growth Factor Receptor 2 (HER2) protein. This new method accurately detects HER2 in serum, offering a promising tool for cancer diagnostics.

Keywords:
AptamerBreast cancerCapacitanceHER2Impedance

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Biosensor Technology

Background:

  • Human Epidermal Growth Factor Receptor 2 (HER2) overexpression is linked to breast cancer proliferation and progression.
  • HER2 is a potential biomarker in serum, but requires sensitive and simple detection platforms.
  • Current detection methods may lack the required sensitivity or simplicity for widespread clinical application.

Purpose of the Study:

  • To develop and optimize a highly sensitive electrochemical platform for HER2 detection.
  • To create a simple, accurate biosensor for quantifying HER2 in biological samples.
  • To explore the utility of DNA aptamers and ferrocene-modified electrodes for HER2 sensing.

Main Methods:

  • Modification of a gold electrode surface with a self-assembled monolayer of DNA aptamer, ferrocenyl hexanethiol, and mercapto hexanethiol.
  • Utilizing Electrochemical Impedance Spectroscopy (EIS) to analyze interfacial capacitance changes.
  • Quantifying HER2 concentration by measuring electrochemical responses in Phosphate-Buffered Saline (PBS) and human serum.

Main Results:

  • Achieved a limit of detection for HER2 as low as 3.61 pg/mL.
  • Demonstrated a sensitivity of 12.28 nF per decade.
  • Established a linear detection range from 1 pM to 1 µM in human serum.
  • Successfully detected HER2 in undiluted human serum, highlighting its practical applicability.

Conclusions:

  • The developed electrochemical aptasensor provides a simple and highly sensitive platform for HER2 detection.
  • This platform shows significant potential as a biomarker for cancer presence and progression.
  • The system is applicable for aptamer screening and studying biological interaction systems.