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Bioremediation of alkane-containing saline soils using the long-chain alkane-degrading bacterium Pseudomonas aeruginosa DL: Effects, communities, and networks.
Tingting Wang1, Jiawei Jing1, Pengfei Huang1
1Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) and Dalian POCT Laboratory, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Journal of Hazardous Materials
|January 28, 2025
View abstract on PubMed
Summary
Pseudomonas aeruginosa DL effectively remediates long-chain hydrocarbon and saline-alkali contaminated soils. Bioaugmentation with this strain significantly enhances soil enzyme activity and promotes beneficial microbial community shifts for improved alkane degradation.
Area of Science:
- Environmental Microbiology
- Bioremediation Science
- Soil Science
Background:
- Soil salinization and long-chain hydrocarbon contamination present significant environmental remediation challenges.
- Developing effective bioremediation strategies for such dual-stressed environments is crucial.
Purpose of the Study:
- To isolate and characterize a bacterial strain capable of degrading long-chain alkanes in saline-alkali soils.
- To evaluate the efficacy of bioaugmentation using the isolated strain for soil remediation.
- To investigate the impact of bioaugmentation on soil enzyme activities and microbial community structure.
Main Methods:
- Isolation and identification of bacterial strain Pseudomonas aeruginosa DL from contaminated soil.
- Assessment of long-chain alkane degradation capacity and saline-alkali tolerance.
- Bioaugmented remediation experiments over 160 days.
- Analysis of soil enzyme activities (dehydrogenase, lipase, catalase, urease).
- Microbial community structure analysis using 16S rRNA sequencing and co-occurrence network construction.
Main Results:
- Pseudomonas aeruginosa DL demonstrated high efficiency in degrading long-chain alkanes (81.36% degradation of initial 30,000 mg/kg).
- Significant increases in soil enzyme activities (dehydrogenase, lipase, catalase, urease) were observed (p < 0.05).
- Successful colonization by P. aeruginosa DL (69% relative abundance) and the emergence of new dominant alkane-degrading genera (Salinimicrobium, Isoptericola, Gordonia, Achromobacter, Ochrobactrum).
- Bioaugmentation streamlined microbial interactions, enhancing associative networks for improved alkane degradation.
Conclusions:
- Pseudomonas aeruginosa DL is a promising candidate for the bioremediation of soils co-contaminated with long-chain hydrocarbons and salinity.
- Bioaugmentation with P. aeruginosa DL effectively restores soil health by increasing enzyme activities and restructuring the microbial community.
- This study provides valuable resources and data for developing effective bioremediation strategies for challenging contaminated soils.
Keywords:
BioaugmentationMicrobial cooccurrence networksPetroleum–contaminated soilPseudomonas aeruginosaSalinization
