Exploring the mechanisms of cadmium tolerance and bioaccumulation in a soil amoeba

Cadmium (Cd) pollution is a global concern. Protists represent a prevalent yet understudied group in soil ecosystems, but our understanding of how protists interact with Cd remains limited. This study investigates the interaction between Cd and the soil amoeba Dictyostelium discoideum, focusing on its resistance, accumulation, and molecular mechanisms. We found that D. discoideum amoebae exhibit strong Cd resistance with an EC50 (half maximal effective concentration) of 899.2 mg/kg and demonstrates significant Cd enrichment capabilities, achieving concentrations up to 1094.70 ± 310.95 mg/kg in stalks and a bioconcentration factor (BCF) of 7.30. Transcriptomic analysis revealed enriched pathways related to DNA replication and identified key genes involved in metal transport, detoxification, and stress response, including abc4, abc16, mms19, gcsA, ucpB, and sodA. Notably, microRNA (miRNA) regulation was found to play a critical role in modulating the expression of these genes. Our findings provide novel insights into the Cd enrichment potential of D. discoideum amoebae and elucidate its mechanisms of heavy metal resistance, highlighting the regulatory role of miRNAs. This study not only advances our understanding of protist-Cd interactions but also opens new avenues for the bioremediation of heavy metal-contaminated soils, where soil amoebae could serve as an effective agent due to their high bioaccumulation factor and rapid growth cycle.