Related Experiment Video
Updated: May 30, 2025

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
Crocin facilitates osteogenesis and angiogenesis by moderating oxidative stress and ferroptosis via Nrf2/GPX4 pathway
Ruilong Hong1, Bo Chen1, Hao Wu1
1Department of Orthopedic Surgery, Xuzhou Central Hospital, Jiangsu 221009, China.
Abstract:
Bone formation is a complex multi-factor process of bone defect healing. Oxidative stress (OS) is predisposed to induce regulatory cell death (RCD), such as ferroptosis. At present, the antioxidant effects of Crocin on erastin induced oxidative damage were studied. The activity of bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) was detected by CCK-8 and EdU staining. The production of reactive oxygen species (ROS), MDA, SOD and GSH were evaluated. Western blotting assay was used to detect ferroptosis-related proteins. The osteogenic function of BMSCs was determined by alkaline phosphatase (ALP) activity, ALP staining and alizarin red S (ARS) staining. Western blotting and RT-PCR assays were used to detect the expression of osteogenic proteins and genes. Angiogenesis of HUVECs was evaluated by tube formation, RT-PCR, scratch test and Transwell assay. The results showed that Crocin can promote the osteogenic function of BMSCs and angiogenesis of HUVECs. In addition, Crocin protects cells from erastin-induced oxidative injury and inhibits ferroptosis via the Nrf2/GPX4 pathway. These findings suggest that Crocin can promote bone defect healing by regulating OS and inhibiting ferroptosis through the Nrf2/GPX4 pathway.
Related Concept Videos
TGF - β Signaling Pathway
Regulation of Angiogenesis and Blood Supply
Necrosis
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
Osteoclasts in Bone Remodeling
Nitric Oxide Signaling Pathway

