Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

52.2K
Overview
52.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extreme diversity of phage amplification rates and phage-antibiotic interactions revealed by PHORCE.

PLoS biology·2025
Same author

Beta-lactamase dependent and independent evolutionary paths to high-level ampicillin resistance.

Nature communications·2024
Same author

Assessing the precision of morphogen gradients in neural tube development.

Nature communications·2024
Same author

Correction to: Distribution of fitness effects of cross-species transformation reveals potential for fast adaptive evolution.

The ISME journal·2023
Same author

Distribution of fitness effects of cross-species transformation reveals potential for fast adaptive evolution.

The ISME journal·2022
Same author

Growth-mediated negative feedback shapes quantitative antibiotic response.

Molecular systems biology·2022
Same journal

Deep learning in tumour genomics: from multi-omics integration to precision oncology.

Open biology·2026
Same journal

Understanding GnRH: local systems, signalling mechanisms and implications in female health.

Open biology·2026
Same journal

The evolution and functional significance of neuropeptide cocktails: insights from SALMFamides in asteroid echinoderms.

Open biology·2026
Same journal

Structural basis of Drosophila insulin receptor activation by DILP2 hormone.

Open biology·2026
Same journal

Parental rearing shapes brain functional networks and socio-sexual behaviours in the prairie vole.

Open biology·2026
Same journal

The periosteum as an endocrine organ: historical foundations and new insights.

Open biology·2026
See all related articles

Related Experiment Video

Updated: May 30, 2025

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.0K

Trace elements increase replicability of microbial growth.

Amit Shimoga Nadig1, Rotem Gross1, Tobias Bollenbach1,2

  • 1Institute for Biological Physics, University of Cologne, Zülpicher Straße 77, Köln 50937, Germany.

Open Biology
|January 28, 2025
PubMed
Summary
This summary is machine-generated.

Supplementing chemically defined media with essential trace elements significantly reduces experimental variability in bacterial growth. This practice enhances precision and replicability in microbiological studies.

Keywords:
growth mediamicrobiology methodsreplicabilityreproducibilitytrace elements

More Related Videos

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader
09:15

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

2.0K
Aseptic Laboratory Techniques: Plating Methods
18:00

Aseptic Laboratory Techniques: Plating Methods

Published on: May 11, 2012

728.6K

Related Experiment Videos

Last Updated: May 30, 2025

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography
06:30

Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli pppGpp Followed by Thin Layer Chromatography

Published on: June 4, 2019

6.0K
Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader
09:15

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

2.0K
Aseptic Laboratory Techniques: Plating Methods
18:00

Aseptic Laboratory Techniques: Plating Methods

Published on: May 11, 2012

728.6K

Area of Science:

  • Microbiology
  • Biochemistry
  • Experimental Design

Background:

  • Chemically defined media often lack essential trace elements.
  • This omission increases experimental sensitivity to trace element contamination.
  • Uncontrolled trace elements introduce variability in growth experiments.

Purpose of the Study:

  • To investigate the impact of trace element supplementation on bacterial growth variability.
  • To demonstrate the benefits of including trace elements in growth media.
  • To advocate for the standardization of trace element use in microbiology.

Main Methods:

  • Cultured 11 bacterial strains in high replicate.
  • Compared growth with and without supplemented trace elements.
  • Maintained all other media conditions identically.

Main Results:

  • Trace element supplementation markedly reduced growth variability across strains.
  • Even in controlled environments, omission of trace elements increased inconsistency.
  • This suggests trace elements are critical for consistent experimental outcomes.

Conclusions:

  • Supplementing trace elements is crucial for reducing variability in chemically defined media.
  • Standardizing trace element use can improve precision and replicability in microbiology.
  • This practice addresses sensitivity to unavoidable contamination.