Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Unlocking Wafer-Scale 3D Photonic Systems With Ion-Beam-Induced Origami.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Nuclear export of R-loop by the DDX1 and XPO1 complex promotes senescence-associated secretory phenotype and inflammaging.

Nature aging·2026

Same author

Doctor-child communication and caregivers' willingness to recommend hospital: a secondary analysis using pediatric patient experience data.

Translational pediatrics·2026

Same author

Stem cell-based therapies for type 1 diabetes: Progress in differentiation, clinical translation, and immune protection.

Animal models and experimental medicine·2026

Same author

Engineering a vascularized-osteogenic microenvironment to enhance bone regeneration via a 3D-printed composite scaffold with progressive-release bio-factors.

Journal of translational medicine·2026

Same author

Identification of communication vulnerabilities in pediatric inpatient admission processes: A failure mode and effects analysis study.

Journal of pediatric nursing·2026

Search research articles

Related Experiment Video

Updated: May 30, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Highly efficient construction of monkey blastoid capsules from aged somatic cells.

Junmo Wu^1,2, Tianao Shao^1,2, Zengli Tang^1,2,3

¹State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.

Nature Communications

|January 28, 2025

Summary

Researchers efficiently generated monkey blastoids, crucial models for early embryonic development, using reprogrammed aged somatic cells. A novel microfluidics platform enhances scalable production for regenerative medicine applications.

More Related Videos

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Related Experiment Videos

Last Updated: May 30, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Area of Science:

Developmental Biology
Stem Cell Biology
Regenerative Medicine

Background:

Blastoids, in vitro blastocyst-like structures, are vital for studying early embryonic development.
Non-human primate blastoids offer an ethical alternative to human models, but low formation efficiency (<30%) has limited their use.
Previous studies primarily used embryonic stem cells, necessitating alternative sources.

Purpose of the Study:

To improve the efficiency and scalability of generating monkey blastoids.
To develop a novel platform for producing blastoid capsules.
To explore the use of reprogrammed aged somatic cells for blastoid generation.

Main Methods:

Generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys.
Utilized a hydrogel-based microfluidics platform for scalable and reproducible blastoid capsule production.
Characterized the size-adjustable, biodegradable blastoid capsules for structural stability and mechanical protection.

Main Results:

Achieved a high blastoid formation efficiency of 80% from reprogrammed aged somatic cells.
Successfully produced scalable, reproducible, size-adjustable, and biodegradable blastoid capsules.
Demonstrated the utility of the microfluidics platform for enhanced blastoid generation.

Conclusions:

High-efficiency generation of monkey blastoids from reprogrammed aged somatic cells is feasible.
The microfluidics platform enables scalable and reproducible production of functional blastoid capsules.
This advancement significantly improves early embryonic development research and holds potential for regenerative medicine.