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Related Experiment Video

Updated: Jun 12, 2025

Acquisition of Resting-State Functional Magnetic Resonance Imaging Data in the Rat
12:41

Acquisition of Resting-State Functional Magnetic Resonance Imaging Data in the Rat

Published on: August 28, 2021

4.2K

fMRI data acquisition and analysis for task-free, anesthetized rats.

Roël M Vrooman1, Monica van den Berg2,3, Gabriel Desrosiers-Gregoire4,5

  • 1Donders Institute for Brain, Behaviour, and Cognition, Nijmegen, The Netherlands.

Nature Protocols
|January 28, 2025
PubMed

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Summary
This summary is machine-generated.

A standardized rat functional MRI (fMRI) protocol ensures reproducible data acquisition across labs. This validated procedure enables large-scale, cross-center comparisons of brain connectivity, crucial for small effect detection.

Area of Science:

  • Neuroimaging
  • Animal Models
  • Magnetic Resonance Imaging

Background:

  • Small animal neuroimaging protocols are often lab-specific, hindering multi-center data comparisons.
  • Standardized protocols are needed to acquire large datasets for robust functional MRI (fMRI) studies.
  • Reproducible data acquisition is key for scientific rigor in the neuroimaging community.

Purpose of the Study:

  • To establish and validate a generalizable protocol for rat functional MRI (fMRI) data acquisition.
  • To facilitate cross-laboratory comparisons of functional connectivity measures.
  • To enable the acquisition of large, high-quality fMRI datasets in rats.

Main Methods:

  • Detailed procedure for rat handling, MRI sequence parameters, and data processing.

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Related Experiment Videos

Last Updated: Jun 12, 2025

Acquisition of Resting-State Functional Magnetic Resonance Imaging Data in the Rat
12:41

Acquisition of Resting-State Functional Magnetic Resonance Imaging Data in the Rat

Published on: August 28, 2021

4.2K
Real-Time fMRI Brain Mapping in Animals
04:05

Real-Time fMRI Brain Mapping in Animals

Published on: September 24, 2020

3.6K
Optogenetic Functional MRI
06:06

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  • Validation across 20 research centers with diverse MRI scanners and field strengths (4.7-17.2 T).
  • Focus on generalizability, quality control, and data analysis.

    • Main Results:

    • A validated, standardized fMRI acquisition protocol for rats.
    • Demonstrated generalizability across multiple institutions and scanner types.
    • Procedure completion time of 1 hour per rat.

    Conclusions:

    • The developed protocol promotes reproducible and comparable fMRI data acquisition in rats.
    • Facilitates large-scale studies and multi-center collaborations in rodent neuroimaging.
    • Accessible to users with limited prior expertise in rat handling or MRI processing.