Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Erratum: Wide-field and non-invasive imaging of brain tumours with scattered light techniques: erratum.

Biomedical optics express·2026

Same author

Predictive Utility of EROA/LVEDV Ratio in Mitraclip Outcomes: Retrospective Multicenter Cohort Study.

Medicina (Kaunas, Lithuania)·2026

Same author

Symbiotic brain-machine drawing via visual brain-computer interfaces.

npj biomedical innovations·2026

Same author

Wide-field and non-invasive imaging of brain tumours with scattered light techniques.

Biomedical optics express·2026

Same author

Fisher information for the design of diffuse optical brain monitoring systems.

Optics express·2026

Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026

Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026

Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026

Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026

Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026

Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 30, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Time-of-flight widefield microscopy.

Khaled Kassem, Ashley Lyons, Philip Binner

|January 29, 2025

Summary

This summary is machine-generated.

We developed a new time-of-flight widefield microscopy method using pseudo-thermal light for high-resolution depth imaging. This technique enhances robustness in noisy conditions and achieves nanometer-scale depth uncertainty.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

Related Experiment Videos

Last Updated: May 30, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

Area of Science:

Optical microscopy
Nanoscale imaging
Biophotonics

Background:

High-resolution depth imaging is crucial for biological microscopy and material science.
Conventional methods like interferometry and holography require phase stability and coherence, limiting performance in noisy or low-light environments.

Purpose of the Study:

To introduce a novel time-of-flight (ToF) widefield microscopy technique.
To overcome limitations of traditional depth imaging methods by enhancing robustness and precision.

Main Methods:

Utilizes pseudo-thermal light source with a standard CMOS camera.
Calculates second-order intensity correlation function, g⁽²⁾, from captured images.
Incorporates a dual-wavelength approach for enhanced depth resolution.

Main Results:

Achieves phase-stable depth imaging, eliminating the need for coherence.
Demonstrates improved robustness in noisy environments.
Reaches nanometer-scale depth uncertainty, validated on biological samples and graphene layers.

Conclusions:

The proposed ToF widefield microscopy offers a robust and precise alternative for depth imaging.
The technique shows significant potential for applications in biological and material sciences requiring high-resolution depth information.