Related Experiment Video
Updated: May 30, 2025
Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
Published on: October 13, 2018
Fertility induction associated with improved peripheral reproductive parameters in male Prop1 mice subjected to GH
Bruna Viscardi Azevedo1, Juliana Moreira Marques1, Nicholas Trigueiro1
1Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo Laboratório de Hormônios e Genética Molecular LIM/42 Divisão de Endocrinologia São PauloSP Brasil Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Laboratório de Hormônios e Genética Molecular LIM/42 - Divisão de Endocrinologia, São Paulo, SP, Brasil.
Objective:
The aim of this study was to characterize the parameters of reproductive anatomy and pituitary hormone expression levels in ames dwarf mice (Prop1 df/df).
Materials And Methods:
Male Prop1 mice aged 30 days received daily intraperitoneal injections of recombinant human GH and levothyroxine three times weekly for 60 days. The sexual maturation of these animals was compared with that of their wild-type (Prop +/+) and untreated (Prop1 ) siblings.
Results:
The Prop1 treated group developed sexual maturation 2 weeks later than the Prop +/+ group and presented an increase in testicular weight, complete spermatogenesis, and enhanced LH and FSH expression. The Prop1 untreated group had low FSH expression and no offspring; most animals in this group did not develop sexual maturation during the study period.
Conclusion:
Replacement with GH and levothyroxine appeared to play a crucial role in restoring peripheral reproductive parameters and increasing pituitary hormone expression in Prop1 mice.
More Related Videos
04:49Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome
Published on: July 5, 2024
05:36Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
Published on: July 7, 2023
