Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advances in analytical methods for regulatory complement proteins in research and clinical applications.

Talanta·2026

Same author

Development of a Sandwich-Type <i>sxtA4</i> Electrochemical Biosensor for Proactive Environmental Monitoring of STX-Producing Microalgae.

Biosensors·2026

Same author

Irregularly Shaped Microplastic Particles Compromise the Integrity of the Glomerular Filtration Barrier of the Kidney.

Kidney360·2026

Same author

His-Tag-Aptamer-Liposome-Conjugates as a Multipurpose Tool for Recombinant Protein Screening and Protein-Based Bioassays.

Analytical chemistry·2026

Same author

Engineering Liposomes and Polymer Conjugates: A Platform for Mechanistic Complement Activation Studies and Controlled Release Applications.

ACS applied materials & interfaces·2026

Same author

Promising early-career (bio)analytical researchers in 2026.

Analytical and bioanalytical chemistry·2025

Search research articles

Related Experiment Video

Updated: May 30, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Studying Cationic Liposomes for Quick, Simple, and Effective Nucleic Acid Preconcentration and Isolation.

Rahel Gruenberger¹, Changyoon Baek², Clemens Spitzenberg¹

¹Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitaetsstr. 31, Regensburg 93053, Germany.

Analytical Chemistry

|January 29, 2025

Summary

A novel vanishing surface strategy using cationic liposomes efficiently captures nucleic acids for pathogen detection. This method improves upon standard kits, offering a simpler, more effective approach for food and water safety testing.

More Related Videos

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Related Experiment Videos

Last Updated: May 30, 2025

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Area of Science:

Biotechnology
Molecular Biology
Environmental Science

Background:

Accurate detection of food and waterborne pathogens is crucial for public health.
Traditional nucleic acid detection methods are limited by inefficient nucleic acid loss during purification.
Existing commercial DNA extraction kits can be cumbersome and less effective.

Purpose of the Study:

To develop a novel "vanishing surface" strategy for efficient nucleic acid capture and purification.
To create a liposome-based method for isolating pathogen DNA and RNA.
To evaluate the efficacy of this new method compared to commercial kits for pathogen detection in various water and food samples.

Main Methods:

Development of cationic liposomes to form lipoplexes with nucleic acids.
Centrifugation and washing of lipoplexes for purification.
Dissolving liposomes in detergent or amplification reactions to release nucleic acids.
Application of the method for DNA isolation from bacteria (E. coli, S. aureus) and adenovirus.
Quantitative PCR (qPCR) for pathogen detection and comparison with a commercial DNA extraction kit.
Concentration of bacteria from larger water volumes using nitrocellulose filters.

Main Results:

The liposome-based method achieved efficient nucleic acid capture and release, with 100% recovery.
Pathogen detection limits as low as 1 CFU or 1 PFU/mL were achieved in buffer.
The novel method demonstrated superior performance over a commercial kit, indicated by lower Ct-values in qPCR.
Effective detection limits were established for various water types and produce rinses (e.g., 100 CFU/100 mL in tap water).

Conclusions:

The vanishing surface strategy using cationic liposomes provides an efficient and homogeneous method for nucleic acid isolation.
This liposome-based protocol is a valuable and simpler alternative to conventional commercial nucleic acid extraction kits.
The method shows significant potential for rapid and sensitive pathogen detection in food and water safety applications.